MTLn3 cells were transfected with cortactin-miRFP703 and EGFP-Tks5101 (link) before plating on gelatin-coated coverslips for 16 h. The cells were imaged every 2 min for 4 h on an IX81-ZDC inverted epifluorescence microscope at 60× magnification (Olympus). Invadopodia lifetimes were quantified manually for at least 30 invadopodia from at least 10 cells per condition in at least 3 experiments. Control and siRNA conditions were imaged on the same day for each experiment. Cells expressing siRNA and scrRNA were identified by co-transfection with siGLO-Red (Dharmacon).
Ix81 zdc inverted epifluorescence microscope
Manufactured by Olympus
The IX81-ZDC is an inverted epifluorescence microscope designed for cell and tissue imaging. It features a motorized Z-drive for precise focus control and a digital camera interface for image capture. The microscope is equipped with a variety of illumination options, including LED and mercury vapor lamps, to enable fluorescence imaging. The core function of the IX81-ZDC is to provide a versatile platform for high-resolution imaging of biological samples.
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2 protocols using ix81 zdc inverted epifluorescence microscope
1
Invadopodia Dynamics Imaged in MTLn3 Cells
2
Quantifying Invadopodia Dynamics in MTLn3 Cells
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MTLn3 cells were transfected with cortactin-miRFP703 and EGFP-Tks5 68 before plating on gelatin-coated coverslips for 16 h. The cells were imaged every 2 min for 4 h on an IX81-ZDC inverted epifluorescence microscope at 60× magnification (Olympus).
Invadopodia lifetimes were quantified manually for at least 30 invadopodia from at least 10 cells per condition in at least 3 experiments. Control and siRNA conditions were imaged on the same day for each experiment. Cells expressing siRNA and scrRNA were identified by co-transfection with siGLO-Red (Dharmacon).
Invadopodia lifetimes were quantified manually for at least 30 invadopodia from at least 10 cells per condition in at least 3 experiments. Control and siRNA conditions were imaged on the same day for each experiment. Cells expressing siRNA and scrRNA were identified by co-transfection with siGLO-Red (Dharmacon).
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