Bbl trypticase soy blood agar tsab plates

The following reagents were provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: MRSA Strain NRS123 (MW2), vancomycin-resistant S. aureus (VRSA) strain NRS281 (CFS-255), and MSSA strain NRS77 (CFS-1155). The CLSI-recommended QC strain S. aureus ATCC 29213 is described as weak beta-lactamase mecA negative (13 ) was obtained from American Type Culture Collection (Manassas, VA). The MRSA strains CFS-2718 and CFS-2720 and MSSA strains CFS-2705 and CFS-2708 are clinical blood culture isolates (USA, 2017). The genome sequence of both ATCC 29213 and MW2 is available (14 (link), 15 (link)). All strains were propagated from frozen stocks by incubation for 24 h at 37°C on BBL Trypticase soy blood agar (TSAB) plates (Becton, Dickinson and Company, Sparks, MD, USA). Exebacase was supplied as 10.64 mg/mL in a liquid carrier solution containing proprietary components (ContraFect Corporation, Yonkers, NY, USA). Vancomycin (catalog no. V8138, lot no. 0000132463) and oxacillin (catalog no. 28221, lot no. BCBJ1695V) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and stock solutions were prepared as described (13 ).

The following reagents were provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: MRSA Strain NRS123 (MW2), vancomycin-resistant S. aureus (VRSA) strain NRS281 (CFS-255), and MSSA strain NRS77 (CFS-1155). The CLSI-recommended QC strain S. aureus ATCC 29213 is described as weak beta-lactamase mecA negative (13 ) was obtained from American Type Culture Collection (Manassas, VA). The MRSA strains CFS-2718 and CFS-2720 and MSSA strains CFS-2705 and CFS-2708 are clinical blood culture isolates (USA, 2017). The genome sequence of both ATCC 29213 and MW2 is available (14 (link), 15 (link)). All strains were propagated from frozen stocks by incubation for 24 h at 37°C on BBL Trypticase soy blood agar (TSAB) plates (Becton, Dickinson and Company, Sparks, MD, USA). Exebacase was supplied as 10.64 mg/mL in a liquid carrier solution containing proprietary components (ContraFect Corporation, Yonkers, NY, USA). Vancomycin (catalog no. V8138, lot no. 0000132463) and oxacillin (catalog no. 28221, lot no. BCBJ1695V) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and stock solutions were prepared as described (13 ).

The S. aureus MRSA strain MW2 (NRS123) was provided on request by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH (30 (link)). Staphylococcus aureus MSSA strain ATCC 29213 was obtained from the American Type Culture Collection (ATCC; Manassas, VA). Strains were propagated from frozen stocks by incubation for 24 h at 37°C on BBL Trypticase soy blood agar (TSAB) plates (Becton, Dickinson and Company, Sparks, MD). Exebacase was supplied at a concentration of 10.64 mg/mL in a liquid carrier solution containing proprietary components (ContraFect Corporation, Yonkers, NY). Daptomycin, vancomycin, and oxacillin were purchased from Sigma-Aldrich (St. Louis, MO), and stock solutions were prepared as described and stored at −20°C prior to use (18 ).