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Thunderbird next sybr qpcr mix reagents

Manufactured by Toyobo
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Sourced in Japan

THUNDERBIRD Next SYBR qPCR Mix reagents are a high-performance real-time PCR reagent mixture designed for quantitative PCR (qPCR) applications. The reagents are optimized for fast and reliable detection and quantification of target DNA sequences using SYBR Green I fluorescent dye.

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Lab products found in correlation

Dnazol, by Molecular Research Center (2 mentions) Steponeplus system, by Thermo Fisher Scientific (2 mentions)

2 protocols using thunderbird next sybr qpcr mix reagents

1

Quantifying OAd-tAIB-Luc in Tumor Tissues

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Total DNA of tumor tissues was isolated using DNAzol (Molecular Research Center, Cincinnati, OH) 18 days after virus administration. The OAd-tAIB-Luc genome copy numbers in the tumor tissues were quantified by real-time PCR analysis using THUNDERBIRD Next SYBR qPCR Mix reagents (TOYOBO, Osaka, Japan) and a StepOnePlus System (Thermo Fisher Scientific). The sequences of the primers are the following: Forward, 5’-GGGATCGTCTACCTCCTTTTG-3’, Reverse, 5’-GGGCAGCAGCGGATGAT-3’.
Ono R., Nishimae F., Wakida T., Sakurai F, & Mizuguchi H. (2022). Effects of pre-existing anti-adenovirus antibodies on transgene expression levels and therapeutic efficacies of arming oncolytic adenovirus. Scientific Reports, 12, 21560.
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2

Quantifying Adenovirus Genome Copy Number

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Pancreatic cancer cells were seeded on a 12-well plate at a density of 1-2×10 5 cells/well. On the following day, cells were infected with OAds at 100 VP/cell. Total DNA was isolated using DNAzol (Molecular Research Center, Cincinnati, OH, USA) 24 and 72 h after infection. The Ad genome copy numbers were quantified by real-time PCR analysis using the primers for the Ad5 E4 and Ad35 E1A genes, a StepOnePlus System (Thermo Fisher Scientific), and THUNDERBIRD NEXT SYBR qPCR Mix reagents (TOYOBO, Osaka, Japan). The sequences of the primers are previously described (27) (link).
Ono R., Takayama K., Onishi R., Tokuoka S., Sakurai F, & Mizuguchi H. (2023). Treatment of Human Pancreatic Cancers Following Local and Systemic Administration of Oncolytic Adenovirus Serotype 35. Anticancer research, 43(2).
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