For IHC, paraffin‐embedded tissue sections were deparaffinized with xylene and rehydrated with an alcohol gradient and water. Sections were incubated with primary antibodies ACAN (diluted 1:500, ab186414, Abcam) at room temperature for 1 h and biotin‐labeled secondary antibodies for 30 min, and then stained with Vectastain ABC kit and DAB peroxidase substrate kit (AR1000, Boster).
Dab peroxidase substrate kit
Manufactured by Boster Bio
Sourced in China
The DAB peroxidase substrate kit is a laboratory reagent used to visualize and detect the presence of peroxidase enzymes in immunohistochemical and immunocytochemical applications. The kit contains a 3,3'-Diaminobenzidine (DAB) chromogen solution that produces a brown insoluble precipitate when catalyzed by the peroxidase enzyme, allowing for the identification and localization of target proteins or cells.
Automatically generated - may contain errors
2 protocols using dab peroxidase substrate kit
1
Immunohistochemical Staining of ACAN
2
Immunohistochemical Analysis of Kidney C3 and IgG
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Kidney sections were cut into 5 μm thick slices. The sections were incubated with primary, rabbit anti-mouse C3 (Abcam, Cambridge, UK), or rabbit anti-mouse IgG (Abcam, Cambridge, UK) antibodies overnight at 4°C and subsequently incubated with HRP-conjugated secondary antibodies. Then, the samples were incubated with the 3,3-diaminobenzidine (DAB) peroxidase substrate kit (Boster, Wuhan, China). The area and integrated option density (IOD) were counted at 400x magnification from 20 high-power fields in each group using an Image-Pro Plus 6.0 photogram analysis system (IPP 6.0, Media Cybernetics, USA).
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