The utilized primary antibodies included mouse monoclonal anti-human insulin and rabbit polyclonal anti-human c-peptide antibodies (Cell Signaling, Denver, USA). The employed secondary antibodies were Alexa Fluor 488-conjugated anti-mouse IgG (H+L) and Alexa Fluor 555-conjugated anti-rabbit IgG (Cell Signaling). The cells were fixed in 4% paraformaldehyde, permeabilized with chilled 100% methanol for 10 min, blocked with 5% normal goat serum for 60 min at RT and incubated with the primary antibodies overnight at 4°C. The cells were then washed with PBS and incubated with the secondary antibodies for 2 hours.
Mouse monoclonal anti human insulin
Manufactured by Cell Signaling Technology
Sourced in United States
Mouse monoclonal anti-human insulin is a primary antibody that specifically binds to human insulin. It is produced by immunizing mice with human insulin and selecting for monoclonal antibodies that recognize this target protein.
Automatically generated - may contain errors
2 protocols using mouse monoclonal anti human insulin
1
Insulin and C-Peptide Immunofluorescence
2
Immunostaining of Pancreatic Hormones
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An Alexa Fluor 488-conjugated rabbit monoclonal antihuman insulin antibody (Cell Signaling Technology, Denver, United States) was used for flow cytometry. The primary antibodies utilized for immunocytochemistry and immunohistochemistry included mouse monoclonal antihuman insulin, rabbit monoclonal anti-human GCG, rabbit polyclonal anti-human c-peptide (Cell Signaling Technology), and rabbit polyclonal antihuman somatostatin (SST) (Novus Biologicals, Littleton, CO). The employed secondary antibodies were Alexa Fluor 488-conjugated anti-mouse IgG (H + L) and Alexa Fluor 555-conjugated anti-rabbit IgG (H + L) (Cell Signaling Technology).
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