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Mai tai sapphire laser

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The Mai Tai Sapphire laser is a mode-locked Ti:Sapphire laser designed for a wide range of applications. It delivers high-energy, ultrashort pulses with a typical pulse duration of less than 100 femtoseconds. The laser operates at a center wavelength of approximately 800 nanometers and provides a tunable output power.

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Lab products found in correlation

Photomultiplier tube, by Hamamatsu Photonics (1 mentions) Plan apochromat, by Zeiss (1 mentions) R3896, by Hamamatsu Photonics (1 mentions)

Close spelling variants of this product

Mai Tai laser (24 citations) Mai Tai Ti:Sapphire lasers (4 citations)

2 protocols using mai tai sapphire laser

1

Two-Photon Imaging of Freely Locomoting Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Head-restrained mice were allowed to freely locomote on a wheel. Imaging was performed with a custom-built two-photon microscope with a resonant scanner. The light source was a Mai Tai Sapphire laser (Spectra Physics) running at 920 nm. The objective was a ×16 CFI LWD Plan fluorite objective water immersion lens with 0.8 NA (Nikon). The detection path consisted of a bandpass filter (525/50 nm) and a 565-nm dichroic mirror directed towards a photomultiplier tube (Hamamatsu). Images were acquired using ScanImage (vidriotechnologies.com)64 (link). Functional images (512 × 32 pixels, 215 × 27 μm2) of Purkinje cell dendrites (50–250 μm below the pia mater) were collected at 283 Hz. Laser power was up to 50 mW at the front aperture of the objective.
Zhang Y., Rózsa M., Liang Y., Bushey D., Wei Z., Zheng J., Reep D., Broussard G.J., Tsang A., Tsegaye G., Narayan S., Obara C.J., Lim J.X., Patel R., Zhang R., Ahrens M.B., Turner G.C., Wang S.S., Korff W.L., Schreiter E.R., Svoboda K., Hasseman J.P., Kolb I, & Looger L.L. (2023). Fast and sensitive GCaMP calcium indicators for imaging neural populations. Nature, 615(7954), 884-891.
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2

Two-Photon Imaging of Purkinje Neuron Activity

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Calcium imaging was acquired with Scanimage 2015 on a commercial two-photon microscope. Excitation light was provided by a Mai Tai Sapphire laser (Spectra-Physics) at 920 nm. Samples were imaged through a 16x objective lens (Zeiss, 20X Plan apochromat, 421452-9900) with a 1:1 mix of deionized water and ultrasound gel (Sonigel, Mettler Electronics) as the immersion medium. Emission light from GCaMP and tdTomato was collected onto green and red channel multi-alkali PMTs (Hamamatsu, R3896), respectively. Images were acquired at 29 Hz with 512 x 512 pixel resolution. Laser power (20–100 mW after the objective) was controlled with Pockels cells (Conoptics). Head-fixed mice were subjected to delay tactile startle conditioning using an ultraviolet LED (Edgelec, 395 nm) as CS and a motorized tactile stimulus as US. A black balloon was fitted over the microscope objective and above the mouse cranial window to block LED light from entering the objective. To visualize Purkinje neuron activity, cells were transduced by AAV-L7-cre and AAV-hSyn1-FLEx-jGCaMP7f. Plcb4+ and Aldoc+ neurons were differentiated by tdTomato expression in Plcb4+ neurons transduced by AAV-Plcb4-tdTomato. Purkinje neuron dendrites were identified as parasagittally elongated structures lying superficial to the Purkinje cell layer.
Chen X., Du Y., Broussard G.J., Kislin M., Yuede C.M., Zhang S., Dietmann S., Gabel H., Zhao G., Wang S.S., Zhang X, & Bonni A. (2022). Transcriptomic Mapping Uncovers Purkinje Neuron Plasticity Driving Learning. Nature, 605(7911), 722-727.
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