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Recombinant mouse icam 1 cd45 fc chimera protein recombinant icam1

Manufactured by R&D Systems

Recombinant mouse ICAM-1/CD45 Fc Chimera Protein is a soluble, recombinant protein consisting of the extracellular domain of mouse ICAM-1 fused to the Fc region of human IgG1. ICAM-1 is a cell adhesion molecule involved in various immune and inflammatory responses.

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2 protocols using recombinant mouse icam 1 cd45 fc chimera protein recombinant icam1

1

OVA257-261 SIINFEKL and Analogues

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OVA257-261 SIINFEKL along with all APL (Q4; SIIQFEKL, Q4R7, SIIKFERL, T4, SIITFEKL, Q4H7, SIIQFEHL, G4, SIIGFEKL) and the NP68 ASNENMDAM were obtained from Cambridge Bioscience. Recombinant mouse ICAM- 1/CD45 Fc Chimera Protein Recombinant ICAM1 was obtained from R&D systems (Cat# 796-IC). For degranulation assays, Rat PE-anti-mouse CD107a (1D4B) (Cat# 12-1071-83) and Rat APC-anti-mouse CD8α (53-6.7) (Cat# 100712) were from Biolegend. Phospho-tyrosine was bound with Mouse Anti-Phosphotyrosine Platinum 4G10 from Merck/Millipore (Cat# 05-1050) and detected by Alexa Fluor 647 donkey anti-mouse (H+L) from Thermo Fisher (Cat# A32787). Alexa Fluor 555-Phalloidin was from Invitrogen (Cat# A34055).
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2

OVA and APL Peptide Evaluation

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OVA257-261 SIINFEKL along with all APLs (Q4, SIIQFEKL, Q4R7, SIIKFERL; T4, SIITFEKL, Q4H7, SIIQFEHL; G4, SIIGFEKL) and the NP68 ASNENMDAM were obtained from Cambridge Bioscience. Recombinant mouse ICAM-1/CD45 Fc Chimera Protein Recombinant ICAM1 was obtained from R&D systems (Cat# 796-IC).
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