Lmd 6500 laser microdissection system

Manufactured by Leica Microsystems

Sourced in Germany

The LMD 6500 Laser Microdissection System is a precision instrument designed for the isolation of specific cells or small tissue samples from larger histological sections. It utilizes a laser beam to cut and capture the selected area of interest for subsequent analysis.

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Lab products found in correlation

Neo clear, by Merck Group (1 mentions) Cresyl violet, by Thermo Fisher Scientific (1 mentions) Ovation picosl wta system v2, by Tecan (1 mentions) Picopure rna isolation kit, by Thermo Fisher Scientific (1 mentions) Bioanalyzer 2100 system, by Agilent Technologies (1 mentions)

2 protocols using lmd 6500 laser microdissection system

Laser Microdissection of Skin Layers

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A Leica LMD 6500 Laser microdissection system (Leica Microsystems, Wetzlar, Germany) was used to isolate the different cell-types from the prepared tissue sections [60 ]. Just before use, the slides with the sections were de-paraffinized with Neoclear^© (Merck, Darmstadt, Germany) for 8–10 min, rinsed in 100% ethanol for one minute and then air-dried. De-paraffinized slides were placed face-down on the microscope and two different cell-type populations (epidermal and hypodermal skin layers) were selected from the berries, microdissected and collected separately. Approximately, 150-100 skin fragments for each cell-type were collected for each of the two independent biological replicates and the pools were brought to a final volume of 50 μL with Pico Pure extraction buffer and processed for RNA extraction.

Balestrini R., Ghignone S., Quiroga G., Fiorilli V., Romano I, & Gambino G. (2020). Long-Term Impact of Chemical and Alternative Fungicides Applied to Grapevine cv Nebbiolo on Berry Transcriptome. International Journal of Molecular Sciences, 21(17), 6067.

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Fibrotic Liver Tissue Isolation and Analysis

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Cryosections from liver tissues at 1 month after BDL and non-treated normal livers (n = 3/3 rats) were stained by Cresyl Violet (Ambion) to visualize the fibrotic septa and surrounding parenchymal tissue regions, which was followed by laser capture microdissection (LCM) using the LMD6500 Laser Microdissection System (Leica Microsystems). By collecting laser-captured liver tissue samples from 5–10 fibrotic septa regions and equally sized regions from surrounding parenchyma of each rat, we isolated 50–150 ng RNA/sample using the PicoPure RNA isolation kit (Life Technology). LCM-derived RNA had a very high integrity without DNA contamination (RIN numbers were between 8.0 and 9.3), as determined using the 2100 Bioanalyzer system (Agilent Technologies). RNA was amplified using the Ovation PicoSL WTA System V2 (NuGEN Technologies). After one round of amplification, at least 7 μg complementary DNA/sample was obtained, subsequently pooled for each group and used for RT-PCR, qRT-PCR analysis for selected genes or PCR array analysis.

Yovchev M.I., Locker J, & Oertel M. (2016). Biliary fibrosis drives liver repopulation and phenotype transition of transplanted hepatocytes. Journal of hepatology, 64(6), 1348-1357.

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