Gum acacia

Standardized extract of B. monniera (CDRI-08 with 55 ± 5% bacosides) (Gifted by Lumen Marketing Company, Chennai, India), was dissolved in 0.5% gum acacia (Hi-Media Laboratories Pvt. Ltd, Mumbai, India). The DNA methyltransferase (DNMT) inhibitor, 5-azacytidine (5-azaC; ≥ 98%, Sigma–Aldrich, India) was dissolved in 0.9% saline. Drugs were prepared freshly on the day of administration (10:00–11:00 h) and drugs were administered to rat pups during their brain growth spurt period (Dobbing and Sands, 1979 (link)). Rat pups from different litters which attained the age of postnatal day (PND)-14 were randomly assigned into three groups: (i) control group (Con, n = 24) received vehicle solution 0.5% gum acacia, per-orally (p.o.) from PND 15–29 and three doses of 0.9% saline intraperitoneally (i.p.) on PND-30 (0, 5, and 23 h), (ii) BME group (BME, n = 24) received 80 mg/kg of CDRI-08 in 0.5% gum acacia (p.o.) from PND 15–29 (Rajan et al., 2011 (link)) and three doses of 0.9% saline (i.p.) on PND-30 (0, 5, and 23 h), (iii) 5-azaC group (5-azaC, n = 24) received 0.5% gum acacia (p.o.) from PND 15–29 and three doses of 3.2 mg/kg of 5-azaC (i.p.) on PND-30 (0, 5, and 23 h) (Sales et al., 2011 (link)).