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Trypsin soy broth

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Trypsin soy broth is a general-purpose microbiological growth medium used for the cultivation of a wide range of bacteria. It provides the essential nutrients and growth factors required for the proliferation of a variety of bacterial species.

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Defiber sheep blood, by BD (1 mentions)

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Trypsin (61 citations)

2 protocols using trypsin soy broth

1

Isolation of Bartonella from Spleens

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Spleens were harvested under sterile conditions from each animal after euthanasia. Approximately 20 mg of each spleen sample was homogenized by adding 200 μL sterilized trypsin soy broth (BD Biosciences, Franklin Lakes, NJ, USA), plated onto two trypsin soy agars containing 5% (vol/vol) defiber sheep blood (BD Biosciences), and incubated at 37 °C in an atmosphere containing 5% CO2. Pure Bartonella colonies were obtained using a protocol described in previous studies19 (link).
Yu J., Li Q., Lu L., Li S., Song X., Li D, & Rao H. (2022). Detection and genetic diversity of Bartonella species in small mammals from the central region of the Qinghai-Tibetan Plateau, China. Scientific Reports, 12, 6996.
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2

Cultivation and Quantification of Porphyromonas gingivalis

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Pg ATCC 33277 was cultured and maintained on enriched trypticase soy agar plates containing 1% yeast extract, 5% defibrinated sheep blood, 5 μg/ml hemin, and 1 μg/ml menadione, at 37°C in an anaerobic atmosphere of 10% H2, 5% CO2, and 85% N2 [32 ]. To prepare Pg for infection, the bacteria were grown in trypsin soy broth (BD Biosciences) containing 1% yeast extract, 5 μg/ml heme and 2.5 μg/ml menadione. The bacteria were collected by centrifugation and washed in PBS, and the number of bacteria (colony-forming units/ml) was determined by measuring the optical density at 600 nm and extrapolating using a standard curve, with a culture of OD600 of 1 equals 109 CFU/ml [32 ].
Zhao Y., Li Z., Su L., Ballesteros-Tato A., Katz J., Michalek S.M., Feng X, & Zhang P. (2020). Characterization and regulation of osteoclast precursors following chronic Porphyromonas gingivalis infection. Journal of leukocyte biology, 108(4), 1037-1050.
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