A pre-treatment consisting of hydrated heating at 121 °C in 10% citrate buffer for 20 min preceded the endogenous peroxidase blocking (DAKO, Glostrup, Denmark) for 5 min and incubation with different primary antibodies: polyclonal IL-1α (1:100, overnight 4 °C; ThermoFisher Scientific, Waltham, MA, USA), polyclonal IL-1RN (1:100, overnight 4 °C; Sigma, St. Louis, MO, USA), monoclonal 8H12 (1:20, overnight 4 °C; ThermoFisher Scientific, Waltham, MA, USA), or polyclonal IFNGR1 (1:1200, overnight 4 °C; ThermoFisher Scientific, Waltham, MA, USA) were used.
Monoclonal 8h12
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Monoclonal 8H12 is a laboratory instrument used for the detection and analysis of specific proteins or molecules in a sample. It utilizes monoclonal antibody technology to capture and identify the target analyte. The core function of the Monoclonal 8H12 is to provide researchers with a reliable and sensitive tool for conducting various immunoassays and protein studies.
Automatically generated - may contain errors
2 protocols using monoclonal 8h12
1
Immunohistochemical Analysis of Inflammatory Markers
2
Immunohistochemical staining of cytokines
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A pre-treatment consisting of hydrated heating at 121 °C in citrate buffer 10% for 20 min preceded the endogenous peroxidase blocking (DAKO, Glostrup, Denmark) for 5 min and incubation overnight 4 °C with different primary antibodies: polyclonal IL-1α (1:100; ThermoFisher Scientific, Waltham, MA, USA), polyclonal IL-1RN (1:100, Sigma, St. Louis, MO, USA), monoclonal 8H12 (1:40; ThermoFisher Scientific, Waltham, MA, USA) or polyclonal IFNGR1 (1:200, ThermoFisher Scientific, Waltham, MA, USA).
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