Polyclonal anti s100b

Lipopolysaccharide from Escherichia coli (LPS, 055:B5), TRI Reagent, minocycline, 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3PO), metformin, oxamic acid, MCC950, fluorocitrate, PFK1 activity assay, anti-S100B (SH-B1), 4-(2-hydroxyethyl) piperazine-l-ethanesulfonic acid (HEPES), o-phenylenediamine (OPD), and HRP- conjugated anti-goat IgG and anti-p38 MAPK were purchased from Sigma (Saint Louis, MO, USA). Arundic acid was purchased from TOCRIS (Bristol, United Kingdom). Standard GFAP was from Calbiochem (San Diego, CA, USA). Polyclonal anti-S100B and polyclonal anti-GFAP were purchased from DAKO (Carpinteria, CA, U.S.A.). The lactate and lactate dehydrogenase (LDH) assays were purchased from BioClin, Brazil. Polyclonal anti-EAAT2 (GLT1) and anti-EAAT1 (GLAST) were purchased from Abcam (Cambridge, MA, USA) and anti-GLUT1, anti-COX2, anti-TLR4 and anti-RAGE were purchased from Santa Cruz Biotechnology (Inc., Dallas, Texas, USA). Monoclonal anti-Iba1 was purchased from Merck/Millipore (Darmstadt, Germany). Anti-phospho p38 MAPK, anti-Akt and phospho Akt (Ser473) were purchased from Cell Signaling Technology (Danvers, Massachusetts, U.S.A.). Anti-HRP conjugated actin was purchased from Proteintech (Rosemento, IL, USA). Finally, HRP-conjugated anti-rabbit IgG and anti-mouse IgG were purchased from GEHealthcare (Little Chalfont, United Kingdom).