Nsg1-Emerald:Addgene cat#54202, Michael Davidson lab; the mutation at residue 114 from D to G was corrected.
Nsg1-mCherry:mouse full length Nsg1 was cloned at the N-terminal of mCherry in pcDNA3 vector.
Nsg2-GFP:mouse full length Nsg2 was cloned into pcDNA-GFP by Genscript
mRFP-Rab5:Addgene cat#14437, Ari Helenius lab.
mCherry Rab5CA(Q79L) cat#35138, mCherry Rab5DN(S34N) cat#35139:Addgene, Sergio Grinstein lab.
EGFP-Rab7 cat#12605, EGFP-Rab7DN(T22N) cat#12660:Addgene, Richard Pagano labmTagBFP2-Rab5, cat#56417, Addgene, Michael Davidson lab;
mTagBFP2-Rab7:Michael Davidson lab.
GFP:Clontech
mCherry:Clontech.