Tba 200fr automated clinical chemistry analyzer

Liver tissues were fixed in 10% neutral buffered formalin for at least 24 h and subsequently embedded in paraffin. Tissue sections (5 μm) were affixed to slides, deparaffinized and stained with hematoxylin & eosin (H&E) to determine morphological changes. For immunohistochemical staining, antigen retrieval was performed using citric acid buffer. The tissue sections were incubated with a primary antibody against NS3 (1:100, orb10789, Biorbyt) overnight. Next, a GTVision^™ III detection system/Mo&Rb was applied (GK500711, Taiwan). Images were acquired using an Olympus BX60 microscope (Japan). Serum biochemistry was evaluated using a TBA-200FR automated clinical chemistry analyzer (Toshiba, Tokyo, Japan).

Serum levels of HBsAg, HBeAg, anti-HBc, and anti-HBs Abs were determined using the AXSYM system kit (Abbott Diagnostika, Wiesbaden, Germany). The cutoff value for determining HBsAg positivity was a signal-to-noise (S/N) ratio of ≥2 and a signal-to-cutoff (S/CO) ratio of ≥1. To detect serum HBV DNA, each serum sample was pretreated with 25 units of DNase I (Roche Diagnostics, Mannheim, Germany) at 37°C overnight, and total DNA was extracted and HBV DNA was detected by real-time PCR [21] (link), [22] (link). Serum alanine transferase (ALT) was measured on a TBA-200FR automated clinical chemistry analyzer (Toshiba, Tokyo, Japan) [21] (link), [22] (link).