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3 protocols using l rhamnose

1

Isolation and Characterization of Polygonatum sibiricum Polysaccharides

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Polygonatum sibiricum were purchased from a local market (Shenyang, Liaoning, China). HepG2 cell line was purchased from Stem Cell Bank, Chinese Academy of Sciences. DEAE-52 cellulose and Sephadex G-100 were obtained from Solarbio Co., Ltd (Beijing China). Monosaccharide standards (D-glucose, D-mannose, D-galactose, L-arabinose, D-xylose, L-rhamnose, and D-fucose) were purchased from Shanghai yuanye Bio-Technology Co., Ltd (Shanghai China). Fetal bovine serum (FBS), Dulbecco's Modified Eagle Medium (DMEM), trypsin EDTA, penicillin and streptomycin were obtained from Gibco (Grand Island, NY, USA). Caspase-3 and caspase-9 kit were purchased from KeyGEN Biotechnology Co., Ltd. (Jiangsu, China). All other chemicals were of reagent grade.
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2

Extraction and Characterization of Polysaccharides

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The BHI medium was purchased from Land Bridge Technology Co., Ltd. (Beijing, China). Chitosan, lactose, galactose, L-rhamnose, and kelp polysaccharide were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Yam peptide and casein peptide were obtained from the food laboratory of Institute of Agricultural Products Processing, Chinese Academy of Agricultural Sciences. Cranberry extract and fucose were purchased from Qingdao Youshun Genesis Technology Co., Ltd. Sialic acid was purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). All solvents and of analytical were analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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3

Extraction and Analysis of C. axillaris Fruit

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C. axillaris fruit was purchased from Ganzhou, Jiangxi Province, China. α-amylase, pepsin, trypsin and monosaccharide standards (L-fucose, L-Fuc; L-rhamnose, L-Rha; L-arabinose, L-Ara; D-glucosamine, D-GlcN; D-glactose, D-Gal; D-glucose, D-Glc; L-xylose, L-Xyl; D-mannose, D-Man; L-fructose, L-Fru; D-galacturonic acid, D-GalA; D-guluronic acid, D-GulA) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China). Pancreatin was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Inulin, resazurin and fatty acid standard (acetic acid, propionic acid, i-butyric acid, n-butyric acid, i-valeric acid, n-valeric acid, n-hexanoic acid) were bought from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). Cysteine, heme chloride, pig bile salt and Tween-80 were from Beijing Solarbio Science & Technology Co.,Ltd. (Beijing, China). Dextran standards (Mw 1152, 5000, 11600, 23800, 48600, 80900, 148000, 273000 Da) were bought from Sigma Chemical Co. Ltd. (St. Louis, MO, USA).
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