Plasma levels of METRNL and irisin were measured using the Enzyme Linked Immunosorbent Assay (ELISA). Plasma samples were thawed on ice and centrifuged for 5 min at 10,000 ×g at 4°C to remove any remaining cells or platelets. Plasma level of METRNL was measured using the Human METRNL ELISA kit (LifeSpan BioSciences, Inc., Seattle, WA, USA. Cat# LS-F13315). The samples were diluted 10× with sample diluent. ELISA was performed according to the manufacturer’s instructions. The intra-assay coefficient of variation was 5.0%–10.0% while the inter-assay coefficient of variation was < 10%. Plasma level of irisin was measured using the irisin recombinant ELISA kit (Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA. Cat #EK-067-29) following the manufacturer’s kit instructions. Plasma samples were diluted 40× with the 1× assay buffer (provided in the kit). The intra-assay coefficient for this ELISA assay was 1.0%–7.0%, while the inter-assay coefficient was < 20%.
Human metrnl elisa kit
Manufactured by LifeSpan BioSciences
Sourced in United States
The Human METRNL ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of METRNL (Meteorin-like protein) levels in human serum, plasma, and other biological fluids. The kit utilizes a specific antibody coated on a 96-well plate to capture the METRNL present in the samples, and a detection antibody is then used to quantify the bound METRNL using a colorimetric reaction. The resulting color intensity is proportional to the METRNL concentration in the sample.
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2 protocols using human metrnl elisa kit
1
Quantifying Plasma Levels of METRNL and Irisin
2
Quantifying Plasma METRNL and Irisin via ELISA
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Plasma levels of METRNL and irisin were detected using an ELISA assay. Plasma samples were thawed on ice and centrifuged for 5 min at 10,000× g at 4 °C to remove any remaining cells or platelets. The plasma level of METRNL was measured using the human METRNL ELISA kit (LifeSpan BioSciences, Inc., Seattle, WA, USA. Cat# LS-F13315). Samples were diluted 10× with sample diluent. ELISA was performed according to the manufacturer’s instructions. The intra-assay coefficient of variation was 5.0–10.0% while the inter-assay coefficient of variation was <10%. Plasma level of irisin was measured using the irisin recombinant enzyme immunoassay kit (Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA. Cat #EK-067-29) following the protocol in the instructions for the kit. Plasma samples were diluted 40× with the 1× assay buffer (provided in the kit). The intra-assay coefficient for this ELISA assay was 1.0–7.0%, while the inter-assay coefficient was <20%.
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