Total microRNA was prepared from the hippocampus and hypothalamus of mice by using ISOGEN II (NipponGene) according to the provided instructions. The microRNA extract was reverse transcribed with the TaqMan MicroRNA Reverse Transcription Kit and TaqMan MicroRNA Assays has-miR-18a and mmr-miR-124a (Life Technologies, Inc., Carlsbad, CA, USA) as described by the manufacturer. Real-time PCR was performed using an ABI PRISM 7900HT Sequence Detection System with TaqMan MicroRNA Assays has-miR-18a (Life Technologies, Inc.). The comparative CT method (ΔΔCt) was used to quantify the relative expression levels of miR-18 and 124a according to the manufacturer's instructions.
Taqman microrna assays has mir 18a
Manufactured by Thermo Fisher Scientific
Sourced in United States
TaqMan MicroRNA Assays has-miR-18a is a product designed for the detection and quantification of the microRNA has-miR-18a. It utilizes real-time PCR technology to accurately measure the expression levels of this specific microRNA.
Automatically generated - may contain errors
1 protocol using taqman microrna assays has mir 18a
1
Quantifying miR-18a and miR-124a Expression
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