Human and mouse aortic tissues or BMDMs were lysed in radioimmunoprecipitation buffer. The protein concentrations were evaluated using a BCA Protein Assay Kit (Thermo Scientific, Waltham, MA). Equal amounts of total protein were separated using 10% SDS‐PAGE and transferred onto polyvinylidene difluoride membranes (Millipore, Billerica, MA). After blocking, the membranes were probed with primary antibodies against CD11b (ab128797, Abcam), p53 (ab26, Abcam), caspase3 (ab1384, Abcam), Bax (ab32503, Abcam), Akt (4691, Cell Signaling Technology, Beverly, MA), p‐Akt (4060, Cell Signaling Technology), β‐actin (ab8226, Abcam), and GAPDH (ab9485, Abcam) at 4℃ overnight. Then, the membranes were incubated with the appropriate horseradish peroxidase–conjugated secondary antibodies. The protein bands were detected by a chemiluminescence detection kit, and β‐actin or GAPDH was used as a loading control. All the experiments were independently conducted in triplicate.
Ab1384
Manufactured by Abcam
Ab1384 is a laboratory equipment product. It is designed for a specific function in the laboratory setting. No further details about its intended use or features are provided in order to maintain an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Ab8226, by Abcam (2 mentions)
Ab32503, by Abcam (2 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Ab128797, by Abcam (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
Ab9485, by Abcam (1 mentions)
Ab692, by Abcam (1 mentions)
Ab97023, by Abcam (1 mentions)
Ab179461, by Abcam (1 mentions)
Ab49822, by Abcam (1 mentions)
Ab6721, by Abcam (1 mentions)
2 protocols using ab1384
1
Western Blot Analysis of Aortic and Macrophage Proteins
2
Investigating Cell Apoptosis and MAPK Pathway in Lung Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blotting was performed to measure the protein levels of cell apoptosis and MAPK pathway proteins in lung tissues, as previously described [24 (link)]. The primary antibodies against the following proteins were used: caspase-3 (1:500, ab1384, Abcam), cleaved caspase-3 (1:500, ab49822, Abcam), Bax (1:3000, ab32503, Abcam), Bcl-2 (1:500, ab692, Abcam), Erk (1:1000, #4694, CST), p-Erk (1:2000, #4370, CST), JNK (1:1000, #4668, CST), p-JNK (1:1,000; ab179461; Abcam), p38 (1;1000, #9212, CST), p-p38 (1:1000, #9211, CST), and β-actin (1:1000, ab8226, Abcam). The following secondary antibodies were used: horseradish peroxidase-conjugated goat anti-mouse IgG H&L (1:2000, ab97023, Abcam) and goat anti-rabbit IgG H&L (1:2000, ab6721, Abcam).
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