Hematoxylin and eosin he

TRIzol reagent was purchased from Invitrogen. ReverTra Ace qPCR RT Master Mix with gDNA Remover Kit and SYBR Green Master Mix were obtained from TOYOBO (Osaka, Japan). Hyaluronidase, Oil Red O, pentobarbital natrium and paraformaldehyde were purchased from Sigma-Aldrich. 3,3′-diaminobenzidine and hematoxylin and eosin (HE) were obtained from Bosterbio. Optimal cutting temperature compound (OCT) was purchased from Sakura Finetek (Torrance, CA, USA). Pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) were obtained from Ningbo Secondary Hormone Corp. (Ningbo, China) . In Situ Cell Death Detection Kit (Fluorescein) was purchased from Roche Applied Sciences. Chemiluminescent HRP Substrate, Embryo max HTF, EmbryoMax M2 medium, Embryo Max KSOM were obtained from Millipore. Oil for embryo culture was purchased from Irvine Science (Santa Ana, CA, USA). Halt Protease & Phosphatase Inhibitor Cocktail was obtained from Pierce. Bicinchoninic acid (BCA) protein assay kit and RIPA lysis and extraction buffer were purchased from Dingguo (Beijing, China). The details, suppliers and dilution of antibodies used in this study are reported in Table 1. All other chemicals were of reagent grade and were obtained from standard commercial sources.