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2 protocols using hyperoside

1

Standardized Phytochemical Compound Acquisition

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Ferulic acid (99%) was purchased from Sigma Aldrich (St. Louis, MO, USA). Ascorbic acid and quercetin (≤100%) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Caffeic acid (≥98%), curcumin (100%), hesperidin (90%), rutin (95.0–101.5%), and triiodothyronine (T3) were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Gallic acid (≥98%), chlorogenic acid (≥95%), ellagic acid (≥95%), sesamin (≥95%), epigallocatechin-3-gallate (≥96%), genistein (≥98%), daidzein (≥95%), luteolin (≥98%), theaflavin (≥98%), isoquercetin (98%), and morin (100%) were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Resveratrol (>99.0%), fisetin (>96.0%), isorhamnetin (>95.0%), and troxerutin (90%) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Cyanidin chloride (99%) was purchased from Nagara Science Co. Ltd. (Gifu, Japan). Apigenin (≥98%) was purchased from LKT Laboratories, Inc. (St. Paul, MN, USA). Kaempferol (98%), myricetin (98%), and naringin (98%) were purchased from BDL Pharmatech Ltd. (Shanghai, China). Hyperoside (90–100%) was purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada). Neohesperidin (≥98%) was purchased from Chem-Impex International, Inc. (Wood Dale, IL, USA). All other chemicals were commercially available and had the highest possible purity.
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2

Analytical Standards for Phytochemical Quantification

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All solvents were obtained from Kanto Chemicals (Tokyo, Japan) in an analytical or HPLC grade. These include methanol, hydrochloric acid 6M (HCl), tertiary butyl hydroxyquinoline (TBHQ) solutions (prepare by dissolving 0.05 g TBHQ in methanol 62.5%), and 0.2% formic acid in distilled water. Purified water was produced in the laboratory using a water distillation system GS-590 device (Advantec, Tokyo, Japan). All pure standard solutions used in this work were prepared with methanol. The standard solutions used in this work are apigenin (purity ≥ 95.0%), myricetin (purity ≥ 96.0%), kaempferol (purity ≥ 97.0%), luteolin (purity ≥ 97.0%), rutin (purity ≥ 94.0%), and quercetin (purity ≥ 95.0%), which were obtained from Cayman Chemical (Ann, Arbor, MI, USA), whereas orientin (purity ≥ 97.0%) and hyperoside (purity ≥ 97.0%) were obtained from Toronto Research Chemical (Canada).
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