Mice were sacrificed, their brains removed and placed on ice. Dorsal striatum and nucleus accumbens were dissected and weighed, then flash-frozen and stored at −80 °C. Tissue samples were ultrasonicated in 0.1 M perchloric acid and stored at −80 °C until extraction. Upon thawing, the samples were homogenized in 0.1 M perchloric acid and centrifuged at 25,000 g for 12 min. Dopamine levels were measured by HPLC with electrochemical detection. The analytical column was a SunFire C18 5 lm (4.6–150.0 mm) from Waters (Milford, MA, USA). The mobile phase was 0.01 M sodium dihydrogen phosphate, 0.01 M citric acid, 1.2 mM sodium EDTA, 1.2 mM sodium 1- heptane sulfonic acid, 10% methanol, pH 3.5; the flow rate was 1.0 mL/min and the column temperature was 34 °C. The installation consisted of a Waters 717 Plus automated injection system, a Waters 1525 Binary pump, and an ESA Coulochem III detector (Dionex, Sunnyvale, CA, USA). Waters Breeze system was used for analysis.
Esa coulochem 3 detector
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ESA Coulochem III detector is a electrochemical detection device used in high-performance liquid chromatography (HPLC) systems. It measures the electrical current generated by the oxidation or reduction of compounds as they pass through an electrochemical cell. The Coulochem III provides sensitive and selective detection of a wide range of analytes.
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2 protocols using esa coulochem 3 detector
1
Dopamine Quantification in Mouse Brain
2
Dopamine Quantification in Mouse Brain Regions
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Mice were sacrificed, their brains removed and placed on ice. Dorsal striatum and nucleus accumbens were dissected and weighed, then flash frozen and stored at -80°C. Tissue samples were ultrasonicated in 0.1 M perchloric acid, and stored at -80°C until extraction. Upon thawing, the samples were homogenized in 0.1 M perchloric acid and centrifuged at 25,000 g for 12 minutes. Dopamine levels were measured by HPLC with electrochemical detection. The analytical column was a SunFire C18 5 lm (4.6 • 150.0 mm) from Waters (Milford, MA, USA). The mobile phase was 0.01 M sodium dihydrogen phosphate, 0.01 M citric acid, 1.2 mM sodium EDTA, 1.2 mM sodium 1heptane sulfonic acid, 10% methanol, pH 3.5; the flow rate was 1.0 mL/min and the column temperature was 34°C. The installation consisted of a Waters 717 Plus automated injection system, a Waters 1525 Binary pump, and an ESA Coulochem III detector (Dionex, Sunnyvale, CA, USA). Waters Breeze system was used for analysis.
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