Mm spm

Manufactured by Bruker

The MM-SPM is a scanning probe microscope designed for materials research. It provides high-resolution imaging and nanoscale characterization of surfaces and thin films. The instrument utilizes multiple scanning probe techniques to collect data about a sample's topography, electrical properties, and other parameters.

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Lab products found in correlation

Nanoscope 5 controller, by Veeco (2 mentions) Scanasyst air probe, by Bruker (2 mentions) Nanoscope analysis version 1.8, by Bruker (1 mentions)

2 protocols using mm spm

Probing Plant Cell Wall Microfibrils

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To probe cellulose microfibrils in cell walls, the primary roots of wild-type and mutant seedlings were subjected to AFM as described previously (Zhang et al. 2019 (link)). The root tips were cut and treated in a peracetic acid solution (11%, v/v) at 85 °C for 3 h. After extensive rinsing, the exposed cell walls of detached root cortex cells were imaged in the air by using a multimode scanning probe microscope (MM-SPM; Bruker) with an advanced NanoScope V Controller (Veeco). All obtained images were scanned in 1-μm scale at 512 × 512 pixels using a ScanAsyst-Air probe (Bruker). The raw images were flattened to remove tilt or bow and then exported in the TIFF format using Nanoscope Analysis (version 1.8; Bruker).

Chen C., Zhang Y., Cai J., Qiu Y., Li L., Gao C., Gao Y., Ke M., Wu S., Wei C., Chen J., Xu T., Friml J., Wang J., Li R., Chao D., Zhang B., Chen X, & Gao Z. (2023). Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots. Plant Physiology, 192(3), 2243-2260.

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Cellulose Nanofiber Orientation Analysis

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The mature second internodes of bc16, bc1, and bc11 mutants, as well as the corresponding wild-type plants, were treated in 11% peracetic acid solution. Fiber cells were isolated from the softening tissues by micromanipulation. The fibers were scanned at a 1-μm scale at 512 × 512 pixels using the ScanAsyst-Air probe on a MultiMode scanning probe microscope (MM-SPM, Bruker) with an advanced NanoScope V Controller (Veeco). At least five different cells from three individual plants were scanned. AFM images were analyzed with SOAX3.6.1 (https://omictools.com/soax-tool) to determine the orientation of cellulosic nanofibers. The cellulosic nanofiber segments detected by SOAX were defined as "snakes" using 1 pixel of snake point space and 20 pixels of minimum snake length. An orientation histogram was calculated based on the snakes after cutting at the junctions. Thousands of snakes were analyzed and shown as a frequency percentage in the histogram (Zhang et al., 2019) .

Xu Z., Gao Y., Gao C., Mei J., Wang S., Ma J., Yang H., Cao S., Wang Y., Zhang F., Liu X., Liu Q., Zhou Y, & Zhang B. (2022). Glycosylphosphatidylinositol anchor lipid remodeling directs proteins to the plasma membrane and governs cell wall mechanics. The Plant cell, 34(12).

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