Human IL-6 and human IL-8 were purchased from Sigma-Aldrich (St. Louis, MO, USA). The following antibodies were purchased from Santa Cruz Biotechnology, Inc., (Santa Cruz, CA, USA): Monoclonal mouse anti-FH (sc-166613), polyclonal goat anti-FHL-1 (sc-17953), monoclonal mouse anti-β-actin (sc-47778), horseradish peroxidase-conjugated donkey anti-goat secondary antibody (sc-2020) and horseradish peroxidase-conjugated goat anti-mouse secondary antibody (sc-2005).
Human il 8
Manufactured by Merck Group
Sourced in United States
Human IL-8 is a laboratory reagent produced by Merck Group. It is a recombinant human Interleukin-8 protein, which is a chemokine involved in the inflammatory response. This product is intended for research use only.
Automatically generated - may contain errors
2 protocols using human il 8
1
Immunoblotting Assay for FH and FHL-1
2
Neutrophil Chemotaxis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Chemotaxis assay was performed as described by Moyes et al. (2009) with minor modifications. In triplicate, 30 μL of either RPMI medium (nonrandom migration) or human IL-8 (Sigma Aldrich Co., 100 ng/ mL, specific migration) was added to the bottom wells of a 48-well chemotaxis chamber (Neuro Probe, Gaith-ersburg, MD). Fifty microliters of cell suspension (3 × 10 6 PMNL/mL) was added to the top of a preloaded filter (Fisherbrand, Thermo Fisher Scientific Inc.) . Plates were incubated for 60 min at 37°C, 5.0% CO 2 , and 95% relative humidity. Five fields per well were counted using a 100× magnification to determine the number of PMNL that migrated in response to IL-8 or RPMI. An average of the 5 fields per well was calculated. For statistical analysis, a chemotaxis index was calculated by subtracting the random migration (RPMI) from the migration with IL-8.
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