Horseradish peroxidase hrp

Boscalid, benalaxyl, fenhexamid, tecloftalam, clothianidin, nitenpyram, imidacloprid, dinotefuran, thiacloprid, thiamethoxam were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Acetamiprid was purchased from Hayashi Pure Chemical Ind., Ltd. (Osaka, Japan). Bovine serum albumin (BSA: Prod. No. A7888) was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Horseradish peroxidase (HRP) was purchased from Toyobo Co., Ltd. (Osaka, Japan). The anti-Boscalid monoclonal antibody (MoAb) was prepared as described previously. 20 The anti-clothianidin MoAb and anti-nitenpyram MoAb were provided from Horiba Ltd. (Kyoto, Japan). 18 All other chemicals and reagents used were of analytical grade, and purchased from Wako Pure Chemical Industries, Ltd. or Nacalai Tesque, Inc. (Kyoto, Japan).

AFB 1 , AFB 2 , AFG 1 and AFG 2 , which were of analytical grade for mycotoxin analysis, and N-hydroxysuccinimide (NHS) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 1-Ethyl-3-(3-dimethyl- aminopropyl)carbodiimide hydrochloride (EDC) was purchased from Dojindo Molecular Technologies, Inc.
(Kumamoto, Japan). Bovine serum albumin (BSA; product no. A7888) was purchased from Sigma-Aldrich Co. LLC (St. Louis, MO, USA). Ninety-six-well microtiter plates (Nunc MaxiSorp) and goat anti-mouse IgG (H＋L) antibody (Pierce) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Horseradish peroxidase (HRP) was purchased from Toyobo Co., Ltd. (Osaka, Japan).
Roasted peanuts produced in Kanagawa prefecture, Japan, were obtained from the market, and it was confirmed by LC-MS/MS that they were not contaminated with AFs. Ground food samples contaminated with AFB 1 , AFB 2 , AFG 1 and AFG 2 were provided by the Food Analysis Performance Assessment Scheme (FAPAS): peanuts (T04118), almond (T04114), pistachio (T04283) and chili powder (T04286). Commercial peanut butter from the USA (17 samples), India (2 samples), or Japan (4 samples) was purchased from markets in the Kansai area of Japan.
All other chemicals and reagents, which were of analytical grade, were purchased from Wako Pure Chemical Industries, Ltd. or Nacalai Tesque, Inc. (Kyoto, Japan).