Ephedrine hydrochloride was purchased by National Institute for the Control of Pharmaceutical and Biological products of China (Beijing, China). Sinapine thiocyanate, tetrahydropalmatine, baicalin and asarinin were purchased from Chengdu Chroma-Biotech Co. Ltd. (Chengdu, China). All compounds except Ephedrine hydrochloride had a purity of 98%; the purity of Ephedrine hydrochloride was 99%.
Glycocholic acid was purchased from Steraloids (Newport, RI, USA) while norepinephrine, epinephrine, alanine serine and valine were obtained from Sigma-Aldrich (St. Louis, MO, USA). HPLC-graded acetonitrile and methanol were obtained from Fisher Scientific (Hampton, NH, USA). Cholic acid D4 and formic acid were purchased from Cambridge Isotope Laboratory Inc. (Cambridge, UK) and Sigma-Aldrich (St. Louis, MO, USA), respectively. Water was purified in-house using a Milli-Q Advantage A10 water purification system (Millipore, Bedford, MA, USA).
Glycocholic acid was purchased from Steraloids (Newport, RI, USA) while norepinephrine, epinephrine, alanine serine and valine were obtained from Sigma-Aldrich (St. Louis, MO, USA). HPLC-graded acetonitrile and methanol were obtained from Fisher Scientific (Hampton, NH, USA). Cholic acid D4 and formic acid were purchased from Cambridge Isotope Laboratory Inc. (Cambridge, UK) and Sigma-Aldrich (St. Louis, MO, USA), respectively. Water was purified in-house using a Milli-Q Advantage A10 water purification system (Millipore, Bedford, MA, USA).