Cells (1 × 10 3 ) were seeded to each well of a 96-well plate. Then, cells were treated by MB-Lipo(SOR)-IL4RTP with or without ultrasound (Sonidel SP-100, Boston, MA, USA). After 48 h, WST-1 solution (5 mg/mL, DoGenBio, Seoul, Republic of Korea) was added to each well. The visible absorbance (at 560 nm) was quantified by a microplate reader (Molecular Devices, Mountain View, CA, USA).
Wst 1 solution
Manufactured by DoGenBio
WST-1 solution is a reagent used in cell proliferation and cytotoxicity assays. It contains a tetrazolium salt that is reduced by metabolically active cells, producing a colored formazan dye. The intensity of the color is directly proportional to the number of viable cells, allowing for the quantification of cell viability and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Microplate reader, by Molecular Devices (1 mentions)
Sorafenib, by Merck Group (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Victortm x3 multilabel plate reader, by PerkinElmer (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Etoposide, by Merck Group (1 mentions)
Sorafenib, by LC Laboratories (1 mentions)
Penicillin, by Corning (1 mentions)
Streptomycin, by Corning (1 mentions)
Imark microplate reader, by Bio-Rad (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
5 protocols using wst 1 solution
1
Evaluating Liposomal Interleukin-4 Therapy
2
Viability Assay for Spheroid Cultures
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To analyze the number of viable cells constituting the spheroids, 7 days after spheroid culture 50 µl of WST-1 solution (DoGenBio, Seoul, South Korea) was added to each well. After 4 h, absorbance at 450 nm was recorded with the help of a microplate reader (BIO-RAD, CA, USA).
3
Cell Viability Assay with Sorafenib and Etoposide
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For the cell viability assays, the cells were seeded in 96-well plates at a density of 5000 cells/well (100 μL total volume/well) and were grown for 24 h. The cells were then washed with sterile phosphate-buffered saline and maintained in low-glucose (5.56 mM) DMEM supplemented with 10% FBS with/without sodium pyruvate (1 mM, Thermo Fisher Scientific, Waltham, MA, USA) for 24 h before adding the sorafenib (LC Laboratories, Woburn, MA, USA) or etoposide (Sigma-Aldrich, Saint Louis, MO, USA). The cells were treated with different concentrations of sorafenib or etoposide, and the controls were treated with vehicle (DMSO, Sigma-Aldrich). After 24-h treatment, WST-1 solution (DoGenBio, Seoul, South Korea) was added to the cells for 1–2 h, and the absorbance at 450 nm was then measured using a VICTORTMX3 Multilabel Plate Reader (PerkinElmer Inc., Waltham, MA, USA).
4
Cell Viability Assay with Metronidazole
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Cells (1×103) were seeded in each well of a 96-well plate and incubated for 18 h at 37°C in a humidified incubator containing 5% CO2 in air. Following incubation, cells were treated with DMSO (0.1%) as a control vehicle and 0, 0.1, 0.2, 0.5, 0.8, and 1 µM indicated concentration of MTZ for 72 h at 37°C. Following this, 20 µl WST-1 solution (DoGenBio, Seoul, Korea) was added to each well for 4 h. The visible absorbance at 460 nm for each well was then quantified using a microplate reader. The assay was repeated 3 times.
5
Cytotoxicity Evaluation of KFD in NIH-3T3 Cells
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Mouse skin fibroblast cells (NIH-3T3) purchased from the Korean Type Culture Collection (KTCC, Seoul, Republic of Korea) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, HyClone, UT, USA) with penicillin (100 units/mL), streptomycin (100 μg/mL) and fetal bovine serum (10%, Corning, NY, USA). To estimate the cytotoxicity of KFD, 1 × 104 cells of NIH-3T3 cells were cultured in a cell culture plate (96-well) and further incubated for 24 h in a CO2 incubator maintained at a constant temperature of 37 °C. After replacing the culture media with serum-free DMEM, KFD (0~100 μM) was added to each well and further incubated for 24 h. WST-1 solution (10 µL/well) (DoGenBio Co., Seoul, Republic of Korea) was added to each well and further incubated during an additional hour in a CO2 incubator. Cell viability was calculated by measuring the absorbance at 450 nm using a microplate reader (iMark Microplate Reader, Bio-Rad Laboratories, Inc. Hercules, CA, USA).
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