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Nr 52725

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The NR-52725 is a laboratory equipment item produced by BEI Resources. It is designed for specific laboratory functions, but a detailed description cannot be provided while maintaining an unbiased and factual approach. Additional information about the core function and intended use of this product is not available.

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Lab products found in correlation

Hek 293t hace2 cell line, by BEI Resources (2 mentions) Vero 76 cells, by American Type Culture Collection (2 mentions) Ccl 136, by American Type Culture Collection (1 mentions) Htb 37, by American Type Culture Collection (1 mentions) Htb 55, by American Type Culture Collection (1 mentions) Ccl 171, by American Type Culture Collection (1 mentions) 293t ace2, by BEI Resources (1 mentions) Hcov oc43, by BEI Resources (1 mentions) Nr 52511, by BEI Resources (1 mentions) Nr 470, by BEI Resources (1 mentions)

3 protocols using nr 52725

1

Coronavirus Cell Culture Maintenance

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The following reagent was obtained through BEI Resources, NIAID, NIH: Human Embryonic Kidney Cells (HEK-293T) Expressing Human Angiotensin-Converting Enzyme 2, HEK-293T-hACE2 Cell Line, NR-52511; SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike-Pseudotyped Lentiviral Kit, NR-52948. Human rhabdomyosarcoma (RD), Vero E6, Huh-7, HEK293T expressing ACE2 (ACE2/293T), and HCT-8 cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM); Caco-2 and MRC-5 cell lines were maintained in Eagle’s Minimum Essential Medium (EMEM). Both media were supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin antibiotics. Cells were kept at 37°C incubator in a 5% CO2 atmosphere. The following reagents were obtained through BEI Resources, NIAID, NIH: Human Coronavirus, OC43, NR-52725; Human Coronavirus, NL63, NR-470. HCoV-OC43 was propagated in RD cells; HCoV-NL63 was propagated in MRC-5 cells. HCoV-229E was obtained from Dr. Bart Tarbet (Utah State University) and amplified in Huh-7 or MRC-5 cells; The Urbani strain of severe acute respiratory syndrome coronavirus (SARS-CoV) and the EMC/2012 strain Middle East respiratory syndrome coronavirus (MERS-CoV) were obtained from the Centers for Disease Control and Prevention. Vero 76 cells were obtained from the American Type Culture Collection.
Hu Y., Ma C., Szeto T., Hurst B., Tarbet B, & Wang J. (2020). Boceprevir, calpain inhibitors II and XII, and GC-376 have broad-spectrum antiviral activity against coronaviruses in cell culture. bioRxiv.
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2

Coronavirus Cell Culture Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following reagent was obtained through BEI Resources, NIAID, NIH: Human Embryonic Kidney Cells (HEK-293T) Expressing Human Angiotensin-Converting Enzyme 2, HEK-293T-hACE2 Cell Line, NR-52511; SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike-Pseudotyped Lentiviral Kit, NR-52948. Human rhabdomyosarcoma (RD), Vero E6, Huh-7, HEK293T expressing ACE2 (ACE2/293T), and HCT-8 cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM); Caco-2 and MRC-5 cell lines were maintained in Eagle’s Minimum Essential Medium (EMEM). Both media were supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin antibiotics. Cells were kept at 37°C incubator in a 5% CO2 atmosphere. The following reagents were obtained through BEI Resources, NIAID, NIH: Human Coronavirus, OC43, NR-52725; Human Coronavirus, NL63, NR-470. HCoV-OC43 was propagated in RD cells; HCoV-NL63 was propagated in MRC-5 cells. HCoV-229E was obtained from Dr. Bart Tarbet (Utah State University) and amplified in Huh-7 or MRC-5 cells; The Urbani strain of severe acute respiratory syndrome coronavirus (SARS-CoV) and the EMC/2012 strain Middle East respiratory syndrome coronavirus (MERS-CoV) were obtained from the Centers for Disease Control and Prevention. Vero 76 cells were obtained from the American Type Culture Collection.
Yanmei H., Chunlong M., Szeto T., Hurst B., Tarbet B, & Wang J. (2021). Boceprevir, calpain inhibitors II and XII, and GC-376 have broad-spectrum antiviral activity against coronaviruses. ACS infectious diseases, 7(3), 586-597.
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3

Diverse Cell Lines for Coronavirus Propagation

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Human rhabdomyosarcoma cell line (RD, ATCC® CCL-136™), African green monkey kidney cell line Vero C1008 (ATCC® CRL-1586™), Human hepatoma cell line Huh-7 (a kind gift from Dr. Tianyi Wang at University of Pittsburgh), and HEK293T expressing ACE2 (293T-ACE2, BEI Resources, NR-52511) cell lines were maintained in Dulbecco’s modified eagle’s medium (DMEM); Human fibroblast cell line MRC-5 (ATCC® CCL-171™), Human lung adenocarcinoma cell line Calu-3 (ATCC® HTB-55™), human Colorectal adenocarcinoma cell line (Caco-2, ATCC® HTB-37™) were maintained in eagle’s minimum essential medium (EMEM, ATCC® 30-2003™). Both media were supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin antibiotics. Cells were kept at cell culture incubator (humidified, 5% CO2/95% air, 37 °C). The following reagents were obtained through BEI Resources, NIAID, NIH: human coronavirus, HCoV-OC43, NR-52725; human coronavirus, HCoV-NL63, NR-470. HCoV-OC43 was propagated in RD cell line; HCoV-NL63 was initially propagated in 293T-ACE2 cell line and accommodated in Vero E6 cell line. HCoV-229E was obtained from Dr. Bart Tarbet (Utah State University) and amplified in Huh-7 or MRC-5 cell lines.
Hu Y., Jo H., DeGrado W.F, & Wang J. (2022). Brilacidin, a COVID‐19 drug candidate, demonstrates broad‐spectrum antiviral activity against human coronaviruses OC43, 229E, and NL63 through targeting both the virus and the host cell. Journal of Medical Virology, 94(5), 2188-2200.
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