Du145 human prostate epithelial cells

Manufactured by American Type Culture Collection

Sourced in United States

DU145 human prostate epithelial cells are a well-characterized cell line derived from a human prostate carcinoma. They are commonly used in research as a model system for studying prostate cancer biology and evaluating potential therapeutic interventions.

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Lab products found in correlation

293ft cell, by Thermo Fisher Scientific (1 mentions) Transwell chamber, by Merck Group (1 mentions) T 75 flasks, by BD (1 mentions) A549 human lung epithelial cells, by American Type Culture Collection (1 mentions)

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2 protocols using du145 human prostate epithelial cells

Culturing Human Epithelial Cell Lines

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HT-29 cf8, a well-differentiated clone of HT-29 human colonic epithelial cells [42 (link),43 (link)] was provided by Dr. Judith M. Ball (College of Veterinary and Biomedical Sciences, Texas A&M University, College Station, TX, USA). DU145 human prostate epithelial cells were obtained from American Type Culture Collection (Manassas, VA, USA), and 293FT cells were obtained from Thermo-Fisher. HT-29 and 293FT cells were grown in DMEM supplemented with 10% FBS, 15 mM HEPES, non-essential amino-acids, and penicillin-streptomycin antibiotic. DU145 cells were grown in RPMI media supplemented with 10% FBS, 5 mM pyruvate, 15 mM HEPES, and penicillin-streptomycin antibiotic. Cells were grown and propagated in T75 flasks and were plated on either 6-well plates, collagen-coated Transwell chambers (Millipore-Sigma), or collagen-coated coverslips for biochemical and functional studies.

Sun L., Cao X., Lechuga S., Feygin A., Naydenov N.G, & Ivanov A.I. (2019). A Septin Cytoskeleton-Targeting Small Molecule, Forchlorfenuron, Inhibits Epithelial Migration via Septin-Independent Perturbation of Cellular Signaling. Cells, 9(1), 84.

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Cell culture protocol for prostate, lung, and colonic epithelial cells

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DU145 human prostate epithelial cells and A549 human lung epithelial cells were acquired from American Type Culture Collection (Manassas, VA). SK-CO15 human colonic epithelial cells were provided by Dr. Enrique Rodriguez-Boulan (Cornell University). DU145 cells were cultured in RPMI medium supplemented with 10% Fetal Bovine Serum, 15% HEPES, pyruvate, and antibiotics. A549 and SK-CO15 cells were cultured in DMEM/F12 and DMEM medium, respectively, supplemented with 10% Fetal Bovine Serum and antibiotics. The cells were grown in T75 flasks (BD Biosciences), and were seeded on collagen-coated coverslips or 6-well plastic plates for immunolabeling and biochemical experiments, respectively.

Wang D., Chadha G.K., Feygin A, & Ivanov A.I. (2015). F-actin binding protein, anillin, regulates integrity of intercellular junctions in human epithelial cells. Cellular and molecular life sciences : CMLS, 72(16), 3185-3200.

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