Hrp conjugated anti mouse igg

Manufactured by Affinity Biosciences

Sourced in United States

HRP-conjugated anti-mouse IgG is a secondary antibody used in various immunoassay techniques. It is produced by conjugating horseradish peroxidase (HRP) to an antibody that specifically recognizes mouse immunoglobulin G (IgG). This conjugate can be utilized to detect and quantify mouse IgG in samples.

Automatically generated - may contain errors

Lab products found in correlation

Sqstm1 p62, by Abcam (1 mentions) Bafilomycin a1 baf a1, by MedChemExpress (1 mentions) Pfoxo1 foxo3a, by Cell Signaling Technology (1 mentions) P ampk, by Affinity Biosciences (1 mentions) Hrp conjugated anti rabbit igg, by Affinity Biosciences (1 mentions) Lamp1, by ABclonal (1 mentions) Il 1β, by Cell Signaling Technology (1 mentions) Interleukin 6 (il 6), by Affinity Biosciences (1 mentions) Lc3a b, by Cell Signaling Technology (1 mentions) Foxo1, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Beclin1, by ABclonal (1 mentions) Tnf α, by ABclonal (1 mentions) Il 18, by ABclonal (1 mentions) Mdck cells, by American Type Culture Collection (1 mentions)

2 protocols using hrp conjugated anti mouse igg

Bafilomycin A1 and Dorsomorphin Dihydrochloride Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bafilomycin A1 (Baf A1) was obtained from MedChem Express (catalog no. HY‐100558, Princeton, USA). Dorsomorphin dihydrochloride (Compound C dihydrochloride) was purchased from MedChem Express (catalog no. HY‐13418, Princeton, USA). Antibodies against p‐AMPK (1:1000; catalog no. AF3423), AMPK (1:1000; catalog no. DF6361), TFEB (1:1000; catalog no. AF7015), IL‐6 (1:1000; catalog no. DF6087) and H3 (1:3000; catalog no. BF9211) were purchased from Affinity Biosciences (OH, USA). LC3A/B (1:1000; catalog no. 4108), IL‐1β (1:1000; catalog no. 12242), GAPDH (1:1000; catalog no. 5174), p‐FOXO1/FoxO3a (1:1000; catalog no. 9464) and FOXO1 (1:1000; catalog no. 2880) were purchased from Cell Signaling Technology (Danvers, MA, USA). LAMP1 (1:1000; catalog no. A16894), ABCA1 (1:1000; catalog no. A16337), IL‐18 (1:1000; catalog no. A16737), Beclin 1 (1:1000; catalog no. A11761), CD36 (1:1000; catalog no. A5792), TNF‐α (1:1000; catalog no. A11534) and CTSD (1:1000; catalog no. A13292) were purchased from ABclonal Technology (Wuhan, China). SQSTM 1/p62 (1:1000; catalog no. ab240635) was purchased from Abcam (Cambridge, the United Kingdom). The secondary antibodies HRP‐conjugated anti‐mouse IgG and HRP‐conjugated anti‐rabbit IgG were purchased from Affinity Biosciences (OH, USA).

Tao J., Yang P., Xie L., Pu Y., Guo J., Jiao J., Sun L, & Lu D. (2021). Gastrodin induces lysosomal biogenesis and autophagy to prevent the formation of foam cells via AMPK‐FoxO1‐TFEB signalling axis. Journal of Cellular and Molecular Medicine, 25(12), 5769-5781.

+ Open protocol

+ Expand

Viral Microneutralization Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

A standard plaque reduction for viral microneutralization was analyzed. In brief, heat-inactivated plasma was cultured with 100 median tissue culture infective doses (TCID₅₀) of each influenza virus in a plate at 37°C in 5% CO₂ for 2 h with or without TPCK-trypsin. MDCK cells (ATCC, Manassas, VA) were added (1.5 × 10⁴ cells/well), cultured for 18-22 h and fixed. The cells were further cultured with anti-influenza A (Bio-Rad, Hercules, CA) or B (Abcam, Cambridge, MA) nucleoprotein mAbs for 1 h and HRP-conjugated anti-mouse IgG (Affinity Biosciences Inc., Amherst, NH) for 1 h. O-phenylenediamine dihydrochloride was then added, and cells were incubated for 5 min. Absorbance was measured at 490 nm. The endpoint titer in triplicates was expressed as the reciprocal of the highest dilution of plasma with an OD value of less than X, where X = [(the average of V wells) − (the average of C wells)]/2 + (the average of C wells).

Luo Z., Ma L., Zhang L., Martin L., Wan Z., Warth S., Kilby A., Gao Y., Bhargava P., Li Z., Wu H., Huang L., Meissner E.G., Li Z., Kilby J.M., Liao G, & Jiang W. (2015). Key Differences in B cell Activation Patterns and Immune Correlates among Treated HIV-Infected Patients Versus Healthy Controls Following Influenza Vaccination. Vaccine, 34(16), 1945-1955.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!