All herbs were provided by the First Affiliated Hospital of Jinan University (China). Blank matrix and RUc (1 g of high or low dose RUc respectively contained 4 g or 2 g herbal drugs) was prepared in the School of Traditional Chinese Medicine, Jinan University.14 (link)-16 (link, link) The effective components and quality control of drugs have been analyzed by HPLC-MS in our previous studies.14 (link)
7,12-dimethylbenz(a)anthracene (DMBA) was purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). Tamoxifen was purchased from Chi-Fei Chemical Co., Ltd (Wuhan, China). The DMEM/F-12 medium, DMEM High Glucose medium, horse serum, fetal bovine serum (FBS) and rhEGF were purchased from Gibco BRL (Grand Island, NY, USA). Matrigel was purchased from BD Biosciences (San Jose, CA, USA). VEGF, MMP2, and MMP9 ELISA kits were purchased from Cusabio Biotech Co., Ltd (Wuhan, China). H-Ras, c-Raf, MEK, p-MEK, ERK, p-ERK, c-jun, and c-fos primary antibodies were purchased from Cell Signaling Techniques (Beverly, MA, USA). VEGF, MMP2, MMP9, CD34, and β-actin were purchased from Abcam (Cambridge, MA, USA).
7,12-dimethylbenz(a)anthracene (DMBA) was purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). Tamoxifen was purchased from Chi-Fei Chemical Co., Ltd (Wuhan, China). The DMEM/F-12 medium, DMEM High Glucose medium, horse serum, fetal bovine serum (FBS) and rhEGF were purchased from Gibco BRL (Grand Island, NY, USA). Matrigel was purchased from BD Biosciences (San Jose, CA, USA). VEGF, MMP2, and MMP9 ELISA kits were purchased from Cusabio Biotech Co., Ltd (Wuhan, China). H-Ras, c-Raf, MEK, p-MEK, ERK, p-ERK, c-jun, and c-fos primary antibodies were purchased from Cell Signaling Techniques (Beverly, MA, USA). VEGF, MMP2, MMP9, CD34, and β-actin were purchased from Abcam (Cambridge, MA, USA).