For cancerous cell proliferation assay, aliquots of HepG2 cells (2 × 104 cells/well) were seeded in 96-well plate, and cultivated in DMEM supplemented with 10% FBS at 37 °C in a humidified atmosphere of 5% CO2. Confluent beating cells were then treated with tested compounds at different concentrations, and imaged using the CloneSelect Imager system (Genetix, UK) after 0, 24, 48, 72 and 96 h treatment. The proliferation curves were drawn with the cell confluence (%) at the indicated time points to evaluate the inhibition effects of compounds on cell proliferation. The assay was performed five times.
Clone select imager system
Manufactured by Genetix
Sourced in United Kingdom
The Clone Select Imager System is a laboratory instrument designed for the visualization and analysis of biological samples. It provides high-quality imaging capabilities for a range of applications, including colony counting, cell viability assays, and gel documentation. The system utilizes a sensitive camera and advanced optics to capture detailed images of samples, enabling users to objectively assess and quantify their experimental results.
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5 protocols using clone select imager system
1
Evaluating Compound Effects on Cancer Cell Proliferation
2
Cell Proliferation Assay using CCK-8
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The CCK-8 kit (Dojindo, China) was adopted to observe cell proliferation. In a 96-well plate, 1 × 103 cells were inoculated in each well and maintained at 37°C. Cell proliferation per well was measured after 0, 24, 48, 72, and 96 h of transfection on a microtiter plate reader (Spectra Rainbow, Tecan) utilizing the Clone Select Imager System (Genetix) as the protocol depicted. All tests were repeated more than 3 times.
3
Cell Viability and Proliferation Assay
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Cell viability was measured with the Cell Counting Kit-8 (CCK-8) (Dojindo, Shanghai, China) according to the manufacturer’s instructions. Cells were plated at a density of 1×103 cells per well in 96-well plates and incubated at 37°C. Proliferation rates were determined at 0, 24, 48, 72 and 96 h post transfection, and quantification was performed on a microliter plate reader (Spectra Rainbow, Tecan) using the Clone Select Imager System (Genetix) according to the manufacturer’s protocol. Values represent the mean ± standard deviation (SD) of four data points from a representative experiment, and experiments were repeated more than three times with similar results. Briefly, transfected cells were plated in six-well plates at a density of 1000 cells per well. The medium was changed every 3 days. After 2 weeks, colonies were fixed with methanol and stained with crystal violet for 20 min. Each experiment was repeated at least three times.
4
Cell Proliferation Assay with CCK-8
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Cell proliferation was measured with the Cell Counting Kit-8 (CCK-8) (Dojindo, China) according to the manufacturer's recommendation. Cells were plated at a density of 2×103 cells per well in 96-well plates and incubated at 37°C. Proliferation rates were determined at 24, 48, 72 and 96h, and the absorbance at 450nm was performed using the CloneSelect Imager System (Genetix).
5
Cell Viability and Colony Formation Assay
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Cell viability was measured with the Cell Counting Kit-8 (CCK-8) (Dojindo, Shanghai, China) according to the manufacturer's instructions. Cells were plated at a density of 1×103 cells per well in 96-well plates and incubated at 37°C. Proliferation rates were determined at 0, 24, 48, 72 and 96 h post-transfection, and quantification was performed on a microtiter plate reader (Spectra Rainbow, Tecan) using the CloneSelect Imager System (Genetix) according to the manufacturer's protocol. Values represent the mean ± standard deviation (SD) of four data points from a representative experiment, and experiments were repeated more than three times with similar results.
Briefly, transfected cells were plated in six-well plates at a density of 1000 cells per well. The medium was changed every 3 days. After 2 weeks, colonies were fixed with methanol and stained with crystal violet for 20 min. Each experiment was repeated at least three times.
Briefly, transfected cells were plated in six-well plates at a density of 1000 cells per well. The medium was changed every 3 days. After 2 weeks, colonies were fixed with methanol and stained with crystal violet for 20 min. Each experiment was repeated at least three times.
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