Accela pda

ESI-MS was performed for the sample from the 300 μM SA-treated fungal culture filtrate. Thermo Scientific MS system (XL/LTQ Orbitrap Discovery) coupled with a Thermo Scientific HPLC system (Accela PDA detector/autosampler/pump) was used. The following conditions were maintained in LC-MS analysis: capillary voltage 45 V, capillary temperature 260°C, auxiliary gas flow rate 10–20 arbitrary units, sheath gas flow rate 40–50 arbitrary units, spray voltage 4–5 kV and a mass range of 100–2000 amu (maximum resolution 30,000). Gradient separation was used on a Waters SunFire C18 RP analytical HPLC column (100Å, 5 μm, 4.6 mm × 150 mm) with a mobile phase of 0–100% MeOH over 30 min at a flow rate of 1 mL min^-1.

UHPLC-HRESIMS analysis was performed using an Exactive^TM Orbitrap mass spectrometer (Thermo Fisher Scientific) equipped with an Accela PDA (Thermo Fisher Scientific) and SEDEX Model 80 LT-ELSD detector (Sedere). The UHPLC was equipped with a Core Shell Kinetex C₁₈ column (2.1 mm × 50 mm, 1.7 μm, Phenomenex), which was operated with a mobile phase flow rate of 0.5 mL/min and a linear gradient from H₂O:CH₃CN (95:5, 0.1% formic acid) at 0.2 min to 100% CH₃CN (0.1% formic acid) at 4.8 min. The mobile phase was held at 100% CH₃CN (0.1% formic acid) for 3.2 min before returning to H₂O:CH₃CN (95:5, 0.1% formic acid) over 0.5 min and equilibrating the column for 1.5 min. The mass spectrometer was operated in positive mode, monitoring a m/z range from 190 to 2000, using a resolution of 30,000, spray voltage of 2.5 kV, capillary temperature of 300°C, tube lens voltage of 145 V, skimmer voltage of 14 V, maximum injection time 50 ms, and microscans of 1. PDA detector was monitoring between 200 and 600 nm.