Cell culture reagents were purchased from Celbio s.r.l. (Milano, Italy). CulturePlate 96/wells plates were purchased from PerkinElmer Life Science (Waltham, MA) and Falcon (BD Biosciences, Bedford, MA). Calcein‐AM, bisBenzimide H 33342 trihydrochloride were obtained from Sigma‐Aldrich (Milan, Italy).
Cell cultures. MDCK‐MDR1, MDCK‐MRP1 and MDCK‐BCRP cells are a gift of Prof. P. Borst, NKI‐AVL Institute, Amsterdam, The Netherlands. Caco‐2 cells were a gift of Dr. Aldo Cavallini and Dr. Caterina Messa from the Laboratory of Biochemistry, National Institute for Digestive Diseases, “S. de Bellis”, Bari (Italy). HT29 and HT29/DX cells from ATCC (Manassas, VA).
MDCK and Caco‐2 cells were grown in DMEM high glucose supplemented with 10 % fetal bovine serum, 2 mM glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, in a humidified incubator at 37 °C with a 5 % CO2 atmosphere.
HT29 cells were grown in RPMI‐1640 supplemented with 10 % fetal bovine serum (FBS), 2 mM glutamine, 100 U/mL penicillin, 100 mg/mL streptomycin; HT29/DX cells were grown in the above mentioned medium containing 50 nM doxorubicin to maintain the chemoresistant phenotype.[32]
Cell cultures. MDCK‐MDR1, MDCK‐MRP1 and MDCK‐BCRP cells are a gift of Prof. P. Borst, NKI‐AVL Institute, Amsterdam, The Netherlands. Caco‐2 cells were a gift of Dr. Aldo Cavallini and Dr. Caterina Messa from the Laboratory of Biochemistry, National Institute for Digestive Diseases, “S. de Bellis”, Bari (Italy). HT29 and HT29/DX cells from ATCC (Manassas, VA).
MDCK and Caco‐2 cells were grown in DMEM high glucose supplemented with 10 % fetal bovine serum, 2 mM glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, in a humidified incubator at 37 °C with a 5 % CO2 atmosphere.
HT29 cells were grown in RPMI‐1640 supplemented with 10 % fetal bovine serum (FBS), 2 mM glutamine, 100 U/mL penicillin, 100 mg/mL streptomycin; HT29/DX cells were grown in the above mentioned medium containing 50 nM doxorubicin to maintain the chemoresistant phenotype.