The concentration of NADPH was measured by EnzyChrom™ NADP+/NADPH assay kit purchased from BioAssay Systems (Hayward, United States). 1×104 BV2 cells were seeded in 24-well plates, and treated with LPS (100 ng/ml) with or without RRx-001 (5 μM). After 24 h, the cells were collected and the samples were prepared according to the manufacturer’s instructions. The absorbance was detected at 565 nm by using infinite M1000 PRO multiscan spectrum (Tecan, Switzerland).
M1000 pro multiscan spectrum
Manufactured by Tecan
Sourced in Switzerland
The M1000 PRO multiscan spectrum is a versatile microplate reader that can measure a wide range of absorbance, fluorescence, and luminescence assays. It features a monochromator-based optical system that provides flexible wavelength selection, enabling various applications in research and diagnostic laboratories.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using m1000 pro multiscan spectrum
1
Measuring NADPH Levels in BV2 Cells
2
Cytotoxicity Evaluation of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse aorta smooth muscle cells (MASMCs) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin, human bronchial epithelial (16HBE) cells were cultured in Keratinocyte Medium (KM) supplemented with 1% keratinocyte growth supplement (KGS) and 1% penicillin–streptomycin. All the cells were cultured at 37 °C in a humidified 5% CO2 incubator.
For cell toxicity assays, the MTT method was adopted. Cells were seeded in a 96-well tissue culture plate, incubated for 12 h, and then treated with different concentrations of compounds. After 48 h of incubation, MTT solution (0.5 mg/ml) was added to the wells. The cells were then incubated for 4–5 h at 37 °C, and formazan crystals in viable cells were dissolved in 100 µl of DMSO. The solubilized formazan was spectrophotometrically quantified with a Tecan M1000Pro Multiscan Spectrum (Tecan) at 490 nm.
For cell toxicity assays, the MTT method was adopted. Cells were seeded in a 96-well tissue culture plate, incubated for 12 h, and then treated with different concentrations of compounds. After 48 h of incubation, MTT solution (0.5 mg/ml) was added to the wells. The cells were then incubated for 4–5 h at 37 °C, and formazan crystals in viable cells were dissolved in 100 µl of DMSO. The solubilized formazan was spectrophotometrically quantified with a Tecan M1000Pro Multiscan Spectrum (Tecan) at 490 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!