The EO of dried samples of C. sempervirens aerial parts was hydrodistilled for 3 h by using a Clevenger apparatus. The obtained (CSEO) was collected and dried over anhydrous sodium sulfate and maintained at 4 °C until analysis.
The analysis of the (CSEO) was carried out on a GC/MS HP model 6980 inert MSD, equipped with an Agilent Technologies capillary HP-5MS column (60 m × 0.25 mm, 0.25 mm film thickness) and coupled to a mass selective detector (MSD5973, ionization voltage 70 eV, all Agilent, Santa Clara, CA, USA). The carrier gas was helium and was used at 1.2 mL/min flow rate. The oven temperature program was as follows: 1 min at 100 °C ramped from 100 to 280 °C at 5 °C/min and 25 min at 280 °C. The chromatograph was equipped with a split/split less injector used in the split less mode. Identification of components was appointed by matching their mass spectra with Wiley Registry of Mass Spectral Data 7th edition (Agilent Technologies) and National Institute of Standards and Technology 05 MS (NIST) library data.
The analysis of the (CSEO) was carried out on a GC/MS HP model 6980 inert MSD, equipped with an Agilent Technologies capillary HP-5MS column (60 m × 0.25 mm, 0.25 mm film thickness) and coupled to a mass selective detector (MSD5973, ionization voltage 70 eV, all Agilent, Santa Clara, CA, USA). The carrier gas was helium and was used at 1.2 mL/min flow rate. The oven temperature program was as follows: 1 min at 100 °C ramped from 100 to 280 °C at 5 °C/min and 25 min at 280 °C. The chromatograph was equipped with a split/split less injector used in the split less mode. Identification of components was appointed by matching their mass spectra with Wiley Registry of Mass Spectral Data 7th edition (Agilent Technologies) and National Institute of Standards and Technology 05 MS (NIST) library data.