Penicillin g streptomycin mix

Lipofectamine™ 2000 transfection reagent, Dulbecco’s modified Eagle minimum essential/Ham’s F-12 (DMEM/F12) plus Glutamax™ media, fetal bovine serum (FBS), sodium pyruvate, L-glutamine, penicillin G/streptomycin mix, RIPA buffer, and enzyme-free PBS-based cell dissociation buffer were purchased from Gibco (Carlsbad, CA, USA). Noble agar, Crystal Violet dye, and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT); non-essential amino acids and protease inhibitor cocktail were acquired from Roche (Palo Alto, CA, USA). β-Mercaptoethanol and rotenone (Rot) were purchased to Sigma-Aldrich (St Louis, MO, USA). Partially reduced graphene oxide (PRGO) was prepared by Abalonyx AS (Oslo, Norway). Graphene oxide (GO) was prepared from natural graphite powder following the modification of the hummers method as follows. An aqueous slurry of GO was dried on a plastic substrate to prepare a GO film. This film was then heated slowly (1 °C/min) to 300 °C in open air to obtain the PRGO film.

Dulbecco's modified Eagle minimum essential/Ham's F-12 (DMEM/F12) plus Glutamax media, Neurobasal medium, minimum essential medium (MEM), B-27 supplement, fetal bovine serum (FBS), sodium pyruvate, l-glutamine, penicillin G/streptomycin mix, and enzyme-free PBS-based cell dissociation buffer were purchased from Gibco (Carlsbad, CA). Staurosporine [ready-made 1 mM solution in dimethyl sulfoxide (DMSO)], l-glutamic acid, d-glucose, uridine, sodium orthovanadate, and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) were acquired from Sigma-Aldrich (St. Louis, MO). Commercially available NNI insecticides were acetamiprid or ACE 200 g/kg soluble powder (SP) in talcum (non-toxic mineral clay) and imidacloprid or IMI 20 g/100 mL of concentrated suspension (CS) in water. They were only used certified brands of insecticides that fulfilled purity standards.