2-hydroxyethylmethacrylate (HEMA), 2-Aminoethyl methacrylate hydrochloride (AEM) N,N′-methylenebisacrylamide (MBAA), ammonium persulphate (APS), 1,2-bis(dimethylamino)ethane (TEMED) and sodium chloride, and cecropin B and cecropin P1 were purchased from Merck KGaA (Darmstadt, Germany). Phosphate buffer saline (PBS) was purchased from Gibco Italia. Peptone, meat extract, tryptone, yeast extract and Noble Agar were purchased from BD Diagnostics (Franklin Lakes, NJ, USA). Antibodies against E. coli (Polyclonal anti-E. coli ab13627) were purchased from Abcam (Cambridge, UK). Quantum dots (QDs) and Qdot™ Incubation Buffer were purchased from Invitrogen (code Q10101 MP and Q20001 MP, respectively).
1 2 bis dimethylamino ethane
Manufactured by Merck Group
Sourced in Germany, Brazil
1,2-bis(dimethylamino)ethane is a chemical compound used as a laboratory reagent. It is a clear, colorless liquid. The compound is commonly used in organic synthesis and chemical research applications.
Automatically generated - may contain errors
Lab products found in correlation
Acrylamide, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (2 mentions)
Meat extract, by BD (1 mentions)
2 hydroxyethyl methacrylate, by Merck Group (1 mentions)
Cecropin p1, by Merck Group (1 mentions)
Qdot incubation buffer, by Thermo Fisher Scientific (1 mentions)
Yeast extract, by BD (1 mentions)
Noble agar, by BD (1 mentions)
Tryptone, by BD (1 mentions)
Peptone, by BD (1 mentions)
Ammonium persulphate, by Merck Group (1 mentions)
Mini protean 3, by Bio-Rad (1 mentions)
Mini protean tetra cell electrophoresis unit, by Bio-Rad (1 mentions)
Dithiothreitol (dtt), by Bio-Rad (1 mentions)
Ammonium peroxydisulfate, by Merck Group (1 mentions)
Terephthalic acid, by Merck Group (1 mentions)
Bis acrylamide, by Merck Group (1 mentions)
Milli q plus system, by Merck Group (1 mentions)
Glacial acetic acid, by RCI Labscan (1 mentions)
6 protocols using 1 2 bis dimethylamino ethane
1
Antibacterial Peptide-Conjugated Quantum Dots
2
SDS-PAGE Protein Analysis Protocol
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Electrophoresis experiments were carried out using a Mini-PROTEAN® 3 or a Mini-PROTEAN® Tetra cell electrophoresis unit (Bio-Rad Laboratories, Inc., USA). The protocols (SDS-PAGE) were carried out according to the manufacturer’s instructions. Reagents of electrophoresis grade were obtained from Bio-Rad Laboratories Inc., electrophoresis grade ammonium persulfate was purchased from Sigma Aldrich and 1,2-bis(dimethylamino)ethane was obtained from Merck Millipore. A broad range of molecular weight markers were purchased from Bio-rad Laboratories Inc. (Precision Plus Protein™ Dual Color Standards) and Thermo Scientific (Scientific PageRuler Plus Prestained Protein Ladder). Unless stated otherwise, samples were prepared following the Laemmli protocol [35 (link)]. Dithiothreitol (DTT, Bio-rad Laboratories Inc.) was selected as the reducing agent. DTT was added to a final 1x concentration of 50 mM. Samples were run on 10% SDS-PAGE vertical minigels under both non-reducing/reducing conditions (DTT included). Electrophoretic conditions were 200 V at constant current according to the manufacturer’s instructions. Minigels were stained with Coomassie blue or silver.
3
Facile Synthesis of Titanium-based Nanocomposites
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Titanium(iv ) isopropoxide (TTIP, 97%) acrylamide (AM, ≥99%), bis-acrylamide (BAM, 99%), ammonium peroxydisulfate (AP, ≥98.0%), 1,2-bis(dimethylamino)ethane (TEMED, 99%), poly(vinyl alcohol) (PVA, 98–99%), polyethylene glycol tert-octylphenylether (triton X-405, 70%), terephthalic acid (TA, 98%), sodium hydroxide (NaOH, 97%) and methylene blue (MB, ≥82%) were purchased from Sigma-Aldrich. Potassium chloride (KCl, 100%) and hydrogen peroxide (H2O2) were obtained from Fischer. Glacial acetic acid (AA, 100%) was purchased from Labscan. Ethanol (analytical grade), n-hexane (95%), acetone (99.50%), 2-propanol (≥99.5%) and LB (Luria–Bertani) nutrient broth were procured from Merck. The cooking oil (sunflower) was of the commercial grade purchased from a local market in Lahore. The Escherichia coli [ACCN, KJ880039] and Staphylococcus aureus [ACCN, KY635411] strains, and deionized water purified by Milli-Q Plus system (Millipore, Bedford, MA, USA) was used for the whole research study.
4
Fabrication of Heat-Responsive Egg White Phantom
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Based on previously described methods [16 (link)], an egg white-based, heat-responsive phantom was fabricated in the study. It was nearly transparent at room temperature. When heated up to 60°C, the phantom became a visibly opaque lesion because of the denaturation and coagulation of egg white protein.
The phantom consisted of 15% acrylamide solution (Sigma-Aldrich, St. Louis, MO), 40% egg white, 44.5% degassed deionized water, and 0.5% ammonium persulfate solution (Sigma-Aldrich). After the mixed solution was degassed for 10 min, 0.2 mL MSNC-PFH nanoemulsion was added and then stirred gently to achieve a uniform distribution. The concentration of droplets was 107 droplets/mL in the phantom. In comparison, the same amount of phosphate buffered saline (PBS) was added as a control (PBS-control) without MSNC-PFH nanoemulsion. Finally, 0.15 mL 1,2-bis(dimethylamino)ethane (Sigma-Aldrich) was added to the entire solution to initiate polymerization. The phantom was kept in a 12°C water bath during polymerization period. The dimensions of each phantom used in the experiments were around 6 × 5 × 3.3 cm.
The phantom consisted of 15% acrylamide solution (Sigma-Aldrich, St. Louis, MO), 40% egg white, 44.5% degassed deionized water, and 0.5% ammonium persulfate solution (Sigma-Aldrich). After the mixed solution was degassed for 10 min, 0.2 mL MSNC-PFH nanoemulsion was added and then stirred gently to achieve a uniform distribution. The concentration of droplets was 107 droplets/mL in the phantom. In comparison, the same amount of phosphate buffered saline (PBS) was added as a control (PBS-control) without MSNC-PFH nanoemulsion. Finally, 0.15 mL 1,2-bis(dimethylamino)ethane (Sigma-Aldrich) was added to the entire solution to initiate polymerization. The phantom was kept in a 12°C water bath during polymerization period. The dimensions of each phantom used in the experiments were around 6 × 5 × 3.3 cm.
5
Enzymatic Hydrolysis of Chlorella Powder
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C. pyrenoidosa powder (CP) used in the experiment was purchased from Jeongwoodang Co., Ltd. (Seoul, Republic of Korea). Microbial protease from Bacillus spp. (EC 3.4.21.14), papain from Carica papaya fruit (EC 3.4.22.2), bromelain from pineapple stem (EC 3.4.22.32), cellulase from Trichoderma reesei (EC 3.2.1.4), and viscozyme L from Aspergillus sp. (cellulolytic enzyme mixture) were purchased from Sigma-Aldrich (St. Louis, MO, USA) (Table 1 ). All other chemicals and reagents were of analytical grade and are listed as follows: sodium dodecyl sulfate, acrylamide, Coomassie brilliant blue, methanol, 1,2-bis (dimethylamino) ethane, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), potassium persulfate, 2,2-diphenyl-1-picrylhydrazyl (DPPH), gallic acid, Folin–Ciocalteu’s phenol reagent, and sodium carbonate were acquired from Sigma-Aldrich. Trichloroacetic acid (TCA) was purchased from Samchun Chemical Co. (Pyeongtaek, Republic of Korea).
6
Protein Extraction from Agaricus bisporus Biomass
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Anhydrous lactose was purchased from Neon, Brazil. Protein extraction from Agaricus bisporus biomass was performed with neutral solutions of tris(hydroxymethyl)aminomethane, monobasic/dibasic sodium phosphate, and sodium chloride, all supplied by LabSynth, Brazil. Other reagents used to treat/preserve protein extracts (2-mercaptoethanol and polyvinylpyrrolidone), in the composition of chromatographic eluents (acetic acid/sodium acetate and glycine), electrolytic solutions (methylene blue and sodium borate), and electrophoretic tests (acrylamide/bisacrylamide, sodium dodecyl sulfate, ammonium persulfate, glycerol, methanol, 1,2-bis(dimethylamino)ethane, dyes, and markers) were purchased from Sigma-Aldrich, Brazil. Chemicals were of analytical grade and used without further purification. All solutions were prepared with ultra-purified water (ρ = 18 MΩ cm−1) from a Millipore Milli-Q system—Brazil.
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