Masson s trichrome

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Masson's trichrome is a histological staining technique used to visualize collagen fibers in biological samples. It primarily stains collagen fibers blue, while other tissue components, such as muscle and cytoplasm, are stained red or pink. This staining method is commonly used in the study of connective tissue and its structural components.

Automatically generated - may contain errors

Lab products found in correlation

Tgf β1, by Bioworld Technology (1 mentions) α sma, by Merck Group (1 mentions) α sma, by Santa Cruz Biotechnology (1 mentions) Vimentin, by Santa Cruz Biotechnology (1 mentions) Tgf β1, by Santa Cruz Biotechnology (1 mentions) P erk, by Santa Cruz Biotechnology (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions) F4 80 bm 8, by Santa Cruz Biotechnology (1 mentions) Hematoxylin eosin, by Merck Group (1 mentions) Masson s trichrome, by Bio-Optica (1 mentions) Myeloperoxidase mpo, by Abcam (1 mentions)

4 protocols using masson s trichrome

Licorice's Impact on NP Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The differences of TGF-β1 (BS1361; Bioworld Technology, Inc., MN, USA), α-SMA (Sigma-Aldrich Corp., St. Louis, MO, USA), vimentin (Cell Signaling), Masson’s trichrome (TASS01; Toson Technology Co., Ltd., Hsinchu, Taiwan), ERK (Cell Signaling), and phosphorylated ERK (p-ERK) (Santa Cruz Biotechnology, TX, USA) staining in those NP specimens before and after licorice treatment were analyzed by immunohistochemistry (IHC).
Furthermore, the method for analyzing TGF-β1, α-SMA, vimentin, Masson’s trichrome, ERK, and p-ERK included selecting three separate full-fields for each patient’s NP specimen under 400× microscopic view and the subsequent use of ImageJ software to filter the stained area. Next, we calculated the ratio of stained area relative to the entire field of view and averaged the nine data of these three patients. Lastly, the differences between the staining biomarkers before and after treatment were analyzed.

Chang G.H., Yang P.R., Cheng Y.C., Hsu K.H., Wu C.Y., Yang Y.H., Lin Y.S., Hsu C.M., Tsai M.S., Tsai Y.T, & Chang P.J. (2022). Nasal irrigation with licorice extract (Glycyrrhiza glabra) in treating nasal polyps by reducing fibroblast differentiation and extracellular matrix production in TGF-β1-stimulated nasal polyp-derived fibroblasts by inhibiting the MAPK/ERK-1/2 pathway – an in vitro and in clinic study. BMC Complementary Medicine and Therapies, 22, 313.

+ Open protocol

+ Expand

Kidney Histopathological Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kidney segments were fixed in 4% formalin for 48 h, then embedded in paraffin and cut. Kidney sections were deparaffinized, rehydrated and stained with hematoxylin & eosin (Sigma-Aldrich, Saint Louis, MO, USA) or Masson’s trichrome (Bio-Optica, Milano, Italy). Fibrotic area quantification of Masson’s trichrome staining was performed as described by Chen et al. using an ImageJ software⁵².
Immunohistochemistry staining was performed as previously described ^{50 (link)}, primary antibodies against F4/80 (BM-8) (diluted 1:30; Santa Cruz biotechnology Inc., Dallas Texas, USA) and Myeloperoxidase (MPO) (diluted 1:50; Abcam, Cambridge, UK) were used for the immunohistochemistry staining. For image processing, Cellsense Entry software (MATIMOP, Tel Aviv, Israel) was used.

Bandach I., Segev Y, & Landau D. (2021). Experimental modulation of Interleukin 1 shows its key role in chronic kidney disease progression and anemia. Scientific Reports, 11, 6288.

+ Open protocol

+ Expand

Histological Analysis of Ureteric Grafts

Check if the same lab product or an alternative is used in the 5 most similar protocols

The reconstructed ureteric segments were xed in 10% buffered formalin. Sections (5 µm) were cut and prepared for histological analysis. Hematoxylin-eosin, Masson's trichrome, and immunohistochemical (IHC) staining using uroplakin-II (UP-II, Santa Cruz, CA, USA) were utilized to reveal the morphology of the grafts, in addition to changes in the epithelium and subepithelial tissue of the LMG. IHC staining using CD31(Santa Cruz, CA, USA) was performed to observe the neovascularization of the LMG.

Xu Y., Sun L., Pan Q., Hua X, & Li B. (2021). A new technique for ureteral reconstruction using lingual mucosa grafts in a beagle model. International urology and nephrology, 53(1).

+ Open protocol

+ Expand

Histological Analysis of Implant Integration

Check if the same lab product or an alternative is used in the 5 most similar protocols

The implants were dissected from the repaired part and horizontal interface of the surrounding tissues and defect area. The samples were fixed in 4% paraformaldehyde, following which they were embedded in paraffin, cut into 5-μm-thick sections, and subjected to Masson’s trichrome staining and immunohistochemical labeling with antibodies against cluster of differentiation CD15 (1:200; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and CD31 (1:200; Millipore, Billerica, MA, USA).
Further, two pathologists who were blinded to the specimens being tested qualitatively analyzed the samples independently. The analysis included an assessment of the extent and type of cellular infiltration and the presence and degree of neovascularization. The number of infiltrated inflammatory cells was determined by numbering the immunopositive cells in six matched microscope fields at × 400 magnification (E600; Nikon, Tokyo, Japan). The neovascularization was assessed based on the immunohistochemical detection of CD31-positive endothelial cells, and a blood vessel was defined as a luminal structure with or without red blood cells. Similarly, the number of blood vessels was counted in six matched microscope fields at × 200 magnification. The tissue areas in the microscope images were calculated from the scale bar.

Yang Z., Song Z., Nie X., Guo K, & Gu Y. (2020). A smart scaffold composed of three-dimensional printing and electrospinning techniques and its application in rat abdominal wall defects. Stem Cell Research & Therapy, 11, 533.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!