Bk fd10p

Manufactured by Biobase

Sourced in China, Belgium

The BK-FD10P is a freeze dryer, a laboratory equipment designed for the process of lyophilization. It is used to remove water from samples through a combination of freezing and vacuum drying. The BK-FD10P has a drying chamber and a refrigeration system to facilitate the freeze-drying process.

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11 protocols using bk fd10p

Evaluating Thermal Stability of Complexes

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The water content of the samples was determined using thermogravimetric analysis (TGA) by measuring the weight loss in the temperature range of 50 to 200 °C.
To assess the stability of the complex, dried samples obtained from a pressure chamber were subjected to lyophilization using a Biobase BK-FD10P, China, freeze dryer. The thermogravimetric analysis (TGA) was performed on all samples using 5 mg of each sample to evaluate the chemical characteristics of the degradation process. The TGA measurements were conducted using an STD 650 (TA Instruments Thermal Analyzer, New Castle, DE, USA). Each sample was heated at a constant rate of 10 °C/min in the presence of air as the reactive gas with a mass flow of 50 mL/min. Additionally, a protective gas of N₂ at a flow rate of 50 mL/min was used in the electronic balance.

Bustos D., Guzmán L., Valdés O., Muñoz-Vera M., Morales-Quintana L, & Castro R.I. (2023). Development and Evaluation of Cross-Linked Alginate–Chitosan–Abscisic Acid Blend Gel. Polymers, 15(15), 3217.

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Preparation of PE-loaded Sphingosomes

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PE-loaded sphingosomes were prepared by the thin-film hydration method with modifications [23 (link)]. The specific weight of the SM and Cholesterol in a weight ratio of (55:45 w/w) was dissolved in 8 mL of chloroform. While the PE (40.2 mg) was dissolved in 2 mL of methanol. Both solutions were then mixed and placed in a round bottom flask. The mixture was evaporated at 65°C in a rotary evaporator (Laborota 4000 efficient, Heidolph, Germany) at 100 rpm under reduced pressure until a dry thin film was obtained on the walls of the flask. Then, 5 mL of PBS (pH 7.4) was added to the film, sonicated for 5 min., and left for 2 hrs for complete hydration. The resultant milky white sphingosomal dispersions of all the formulas were then lyophilized using a BK-FD10P freeze drier (Biobase, China). The system was cooled to—46 C°, under a vacuum pressure of 1pa for 36 hrs. The dried powdered samples were then stored at 4 ± 2°C and utilized for further investigations.
The compositions of the prepared formulations are shown in Table 1 and the procedure of the preparation is summarized in Fig 2.

AlMadalli H.J., Abdul Rasool B.K., Shehab N.G., Sala F.D, & Borzacchiello A. (2024). Pomegranate extract-loaded sphingosomes for the treatment of cancer: Phytochemical investigations, formulation, and antitumor activity evaluation. PLOS ONE, 19(2), e0293115.

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Punica granatum L. Pericarp and Seed Extraction

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The fresh fruit’s pericarps and seeds of Punica granatum L were separated and weighed (1.49 kg and 1.3 kg, respectively). The pericarps were then air-dried and powdered. The powder was divided equally and was macerated exhaustively by cold maceration using absolute ethanol (3L) and distilled water (1L), while the seeds were mixed separately with absolute ethanol (2L) and distilled water (1L) using a blender. All extracts were filtered. The ethanolic extracts were separately evaporated at 60°C under reduced pressure, using a rotary evaporator.
On the other hand, the aqueous extracts were freeze-dried at -46 C°, under 1 pa, and for 36 hrs using a lyophilizer (BK-FD10P, Biobase, China). All extracts were stored in the fridge at 4°C for further analysis.

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Extraction and Analysis of Plant Metabolites

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Air-dried powdered aerial parts (50 g) were extracted with 80% MeOH (1:20 w/v) by sonication (100 kHz, ultra-sound bath Biobase UC-20C) for 15 min (×2) at room temperature. Then, the methanol was evaporated in vacuo and water residues were lyophilized (lyophilizer Biobase BK-FD10P) to yield crude extract 1.38 g. Afterwards, the lyophilized extract was dissolved in 80% methanol (0.1 mg/mL), filtered through a 0.45 μm syringe filter (Polypure II, Alltech, Lokeren, Belgium), and an aliquot (2 mL) of each solution was subjected to UHPLC–HRMS analyses. The same extract was used for further in vitro and in vivo tests.

Gevrenova R., Kostadinova I., Stefanova A., Balabanova V., Zengin G., Zheleva-Dimitrova D, & Momekov G. (2023). Phytochemical Profiling, Antioxidant and Cognitive-Enhancing Effect of Helichrysum italicum ssp. italicum (Roth) G. Don (Asteraceae). Plants, 12(15), 2755.

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Extraction of Plant Metabolites

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Air-dried aerial parts (100 g) were extracted twice with 80% MeOH (1:20 w/v) by sonication (80 kHz, ultra-sound bath Biobase UC-20C) for 15 min at room temperature. The extracts were concentrated in vacuo and subsequently lyophilized (lyophilizеr Biobase BK-FD10P) to yield crude extracts of 8.125 g.

Mihaylova R., Gevrenova R., Stefanova A., Zheleva-Dimitrova D., Balabanova V., Zengin G., Simeonova R, & Momekov G. (2023). The Phytochemical Profiling, In Vitro Antioxidant, and Hepatoprotective Activity of Prenanthes purpurea L. and Caffeoylquinic Acids in Diclofenac-Induced Hepatotoxicity on HEP-G2 Cells. International Journal of Molecular Sciences, 24(18), 14148.

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Extraction and Analysis of Medicinal Plant Metabolites

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Air-dried powdered leaves (50 g) and flowering heads (10 g) were extracted with 80% MeOH (1:20 w/v) by sonication (100 kHz, ultra-sound bath Biobase UC-20C) for 15 min (×2) at room temperature. The methanol was evaporated in vacuo, and water residues were lyophilized (lyophilizer Biobase BK-FD10P) to yield crude extracts as follows: leaves—10.67 g and flowering heads—0.90 g. Then, the lyophilized extracts were dissolved in 80% methanol (0.1 mg/mL), filtered through a 0.45 μm syringe filter (Polypure II, Alltech, Lokeren, Belgium), and an aliquot (2 mL) of each solution was subjected to UHPLC–HRMS analyses. The same extracts were used for pharmacological tests.

Zheleva-Dimitrova D., Petrova A., Zengin G., Sinan K.I., Balabanova V., Joubert O., Zidorn C., Voynikov Y., Simeonova R, & Gevrenova R. (2023). Metabolite profiling and bioactivity of Cicerbita alpina (L.) Wallr. (Asteraceae, Cichorieae). Plants, 12(5), 1009.

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Chitosan-Based Nanocapsules for HT Encapsulation

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Chitosan solution (1.5% w/v) was prepared by dissolving chitosan in 1% (v/v) glacial acetic acid solution. After complete dissolution, the solution was centrifuged at 4000 rpm for 5 min to clear any heterogeneous impurities in the solution. Simultaneously, 0.75% (w/v) solution of sodium bisulfate was prepared in double distilled water. A 1 mg/mL solution of HT was prepared in double distilled water and added drop by drop to chitosan solution under continuous stirring using a magnetic stirrer. The stirring was continued for 10 min before adding the sodium bisulfate solution slowly into the chitosan-HT mixture and the stirring was continued for 1 h at ambient temperature (25 ± 1 °C). Finally, the solution containing HT nanocapsules was ultrasonicated for 5 min at 40 kHz and frozen to −18 °C before subjecting it to freeze drying (BIOBASE, BK-FD10P). The control sample was prepared without including HT in the nanocapsules. The HTS1 and HTS2 samples included 5 mg/g and 20 mg/g of HT in the finally dried nanoencapsulated powders, respectively. All the freeze dried samples were sealed in air tight containers and stored at −18 ℃ till further analysis.

Ahmed Wani T., Masoodi F.A., Akhter R., Akram T., Gani A, & Shabir N. (2021). Nanoencapsulation of hydroxytyrosol in chitosan crosslinked with sodium bisulfate tandem ultrasonication: Techno-characterization, release and antiproliferative properties. Ultrasonics Sonochemistry, 82, 105900.

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Spectroscopic Analysis of CXB-Loaded SA Gel

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Samples (10 mg) of CXB powder, SA powder, CXB/SA physical mixture, and freeze-dried gel were loaded into a Shimadzu FTIR spectroscope (IRAffinity-15, Japan), and their spectra were recorded over a wave range of 450-4000 cm⁻¹. The CXB-loaded SA gel was dried by a Biobase Freeze Dryer (BK-FD10P, China) under the conditions of -59°C and 0.001 mp vacuum pressure for 24 h.
The CXB/SA physical mixture was prepared by continuous mixing in a mortar and pestle of CXB with the polymer at a molar ratio of 1 : 1 w/w for 30 min. FTIR spectroscopy analysis was performed to study the possibility of the molecular interaction between CXB and SA.

Abdul Rasool B.K., Khalifa A., Abu-Gharbieh E, & Khan R. (2020). Employment of Alginate Floating In Situ Gel for Controlled Delivery of Celecoxib: Solubilization and Formulation Studies. BioMed Research International, 2020, 1879125.

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Phytochemical Extraction and Analysis

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Air-dried powdered leaves and flowering heads (100 g) were extracted twice with 80% MeOH (1:20 w/v) by sonication (80 kHz, ultrasound bath Biobase UC-20C) for 15 min at room temperature. The extracts were concentrated in vacuo and lyophilized (lyophilizer Biobase BK-FD10P) to yield crude extracts of 5.10 g (leaves) and 4.51 g (flowering heads). The lyophilized extracts were dissolved in 80% methanol (0.1 mg/mL). An aliquot (2 mL) of each extract solution was filtered through a 0.45 μm syringe filter (Polypure II, Alltech, Lokeren, Belgium) and subjected to UHPLC–HRMS analyses.

Zheleva-Dimitrova D., Simeonova R., Kondeva-Burdina M., Savov Y., Balabanova V., Zengin G., Petrova A, & Gevrenova R. (2023). Antioxidant and Hepatoprotective Potential of Echinops ritro L. Extracts on Induced Oxidative Stress In Vitro/In Vivo. International Journal of Molecular Sciences, 24(12), 9999.

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Extraction and Analysis of Plant Compounds

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Air-dried powdered roots, aerial parts (stems and leaves), and flower heads (15 g) were extracted with 80% MeOH (1:20 w/v) by sonication (80 kHz, ultra-sound bath Biobase UC-20C) for 15 min (×2) at room temperature. Then, the extracts were concentrated in vacuo and lyophilized (lyophilizer Biobase BK-FD10P) to yield crude extracts as follows: flower heads 3.54 g, aerial parts 2.82 g and roots 2.14 g. The lyophilized extracts (1 mg) were dissolved in 80 % methanol (10 mL). An aliquot (2 mL) of each extract solution was filtered through a 0.45 μm syringe filter disc (Polypure II, Alltech, Lokeren, Belgium) and subjected to UHPLC–HRMS analyses.3.3. Chemicals
Acetonitrile and formic acid for LC–MS, and HPLC grade methanol were purchased from Fisher Scientific (Hampton, NY, USA).
The authentic standards used for compound identification were obtained as follows: gentisic acid, vanillic acid, protocatechuic acid, quercetin, luteolin, apigenin, genkwanin, apigenin 7-O-glucoside, kaempferol 3-O-glucoside, luteolin 7-O-glucoside and kaempferol 3-O-rutinoside, from Extrasynthese (Genay, France); caffeic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid and hispidulin were supplied from Phytolab (Vestenbergsgreuth, Germany); chlorogenic acid acaciin and pectolinarin were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Zheleva-Dimitrova D., Zengin G., Ak G., Sinan K.I., Mahomoodally M.F., Gevrenova R., Balabanova V., Stefanova A., Nedialkov P, & Voynikov Y. (2021). Innovative Biochemometric Approach to the Metabolite and Biological Profiling of the Balkan Thistle (Cirsium appendiculatum Griseb.), Asteraceae. Plants, 10(10), 2046.

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