Hla dr pe

Manufactured by Sony

Sourced in United States, Germany

The HLA-DR-PE is a laboratory reagent used in flow cytometry applications. It consists of a monoclonal antibody specific for the HLA-DR antigen, conjugated to the fluorescent dye R-Phycoerythrin (PE). This product is intended for research use only and its core function is to enable the identification and analysis of HLA-DR-expressing cells in biological samples.

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Lab products found in correlation

Cd107a pe cy5, by Thermo Fisher Scientific (1 mentions) Cd56 apc, by Miltenyi Biotec (1 mentions) Cd2 pe cy7, by Sony (1 mentions) Cd56 brilliant violet 421, by Sony (1 mentions) Cd57 fitc clone tb03, by Miltenyi Biotec (1 mentions) Cd56 pe, by Agilent Technologies (1 mentions) Sytox blue dead cell stain, by Thermo Fisher Scientific (1 mentions) Cd56 apc, by Beckman Coulter (1 mentions) Cd14 pe vio770, by Miltenyi Biotec (1 mentions) Cd45 vioblue, by Miltenyi Biotec (1 mentions) Pd 1 alexa fluor 647, by BioLegend (1 mentions) Cd45ra apc, by Sony (1 mentions) Cd197 fitc, by Sony (1 mentions) Cd16 apc, by Miltenyi Biotec (1 mentions) Cd56 apc vio770, by Miltenyi Biotec (1 mentions) Cd3 vioblue, by Miltenyi Biotec (1 mentions)

2 protocols using hla dr pe

Multiparameter Flow Cytometry for NK Cells

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For surface and intracellular staining of NK cells the following mouse anti-human fluorescent-labeled Abs were used: CD2-PE-Cy7 (clone TS1/8), CD56-BrilliantViolet421 (clone HCD56), CD56-AF488 (clone 5.1H11), CD56-PE-Cy7 (clone 5.1H11), CD57-PE (clone TB01), HLA-DR-FITC (clone LN3), HLA-DR-PE (clone L243), IFN-gamma-FITC (clone 4S.B3), KI-67-PE (clone Ki-67), NKp46-FITC (clone 9E2) (Sony Biotechnology, USA), CD16-PE (Sorbent, Russia), CD16-APC (clone REA423), CD56-APC (clone REA196), CD57-FITC (clone TB03), CD57-APC (clone TB03), CD57-PE-Vio770 (clone TB03) (Miltenyi Biotec, Germany), CD44-F (clone J.173, Immunotech, USA), CD56-PE (clone C5.9, Dako, USA), CD107a-PE-Cy5 (clone H4A3), NKp30-PE (clone P30-15) (eBioscience, USA), CD161-AlexaFluor647 (clone HP-3G10), Granzyme B-Alexa Fluor 647 (clone GB11), KIR2DL2/DL3-PE (clone DX27) (Biolegend, USA), FcεRIγ-FITC (polyclonal, Milli-Mark, Merck, Germany), NKG2A-PE (clone 131411), NKG2C-AlexaFluor 488 (clone 108724), NKG2C-PE (clone 134591) (R&D Systems, USA). Supernatant of hybridoma producing Abs to human KIR2DL1 was kindly provided by prof. Miguel Lopez-Botet (Universitat Pompeu Fabra, Barcelona, Spain). Sheep anti-mouse IgG-FITC and PE (Sigma-Aldrich, USA) were used as second Abs for anti-KIR2DL1. IgG1-PE (clone IS5-21F5, Miltenyi Biotec, Germany) was used as isotype control for NKG2C-PE Ab staining.

Kobyzeva P.A., Streltsova M.A., Erokhina S.A., Kanevskiy L.M., Telford W.G., Sapozhnikov A.M, & Kovalenko E.I. (2020). CD56dimCD57−NKG2C+ NK cells retaining proliferative potential are possible precursors of CD57+NKG2C+ memory-like NK cells. Journal of leukocyte biology, 108(4), 1379-1395.

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Comprehensive Immune Phenotyping of PBMC Samples

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PBMC samples were stained with the panels of fluorochrome-conjugated monoclonal antibodies, which are in the Supplementary Table S1. The list of mouse anti-human antibodies included: NKG2C-FITC (4:100, clone REA205), CD3-Vioblue (0.4:100, clone BW264/56), CD56-APC-Vio770 (clone REA196), CD45-VioBlue (2:100, clone REA747), CD14-PE-Vio-770 (0.4:100, clone TÜK4), CD16-APC (3:100, clone REA423) from Miltenyi Biotec, Bergisch Gladbach, Germany; HLA-DR-PE (3:100, clone L243, Sony Biotechnology, San Jose, CA, USA), CD3-PerCP (5:100, clone HIT3a), CD3-FITC (3:100, clone FIT3a), CD45RA-APC (2:100, clone HI100), CD197-FITC (5:100, clone G043H7), KIR2DL2/L3- APC (1:100, clone DX27), NKp30-PE (3:100, clone P30-15), NKG2D-PE (3:100, clone 1D11), CD45-PerCP (2:100, clone 2D1), CD57-APC (4:100, clone HNK-1) from Sony Biotechnology, San Jose, CA, USA; CD56-APC (3:100, clone N901) from Beckman Coulter, Brea, CA, USA, PD-1-Alexa Fluor 647 (1:100, clone EH12.2H7) from BioLegend, San Diego, CA, USA.
Viability staining with SytoxBlue Dead Cell stain was performed (Invitrogen, Waltham, MA, USA). The gating strategy is presented in Figure 1.

Vavilova J.D., Ustiuzhanina M.O., Boyko A.A., Streltsova M.A., Kust S.A., Kanevskiy L.M., Iskhakov R.N., Sapozhnikov A.M., Gubernatorova E.O., Drutskaya M.S., Bychinin M.V., Novikova O.N., Sotnikova A.G., Yusubalieva G.M., Baklaushev V.P, & Kovalenko E.I. (2023). Alterations in the CD56− and CD56+ T Cell Subsets during COVID-19. International Journal of Molecular Sciences, 24(10), 9047.

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