Dimethyl sulfoxide dmso

PHE, NP, NP-1 and PMMA were purchased from FUJIFILM Wako Pure Chemical (Osaka, Japan). ANT-1 was purchased from Combi-Blocks (San Diego, USA). ANT, ANT-2, NP-2, BP and COR were purchased from Tokyo Chemical Industry (Tokyo, Japan). PHE-2 and PHE-3 were purchased from Merck-Sigma-Aldrich Japan (Tokyo, Japan). Spectroscopic grade solvents (CHCl₃, THF, cyclohexane (CHX), and dimethyl sulfoxide (DMSO)) were purchased from Dojindo Laboratories (Kumamoto, Japan).

Recombinant human MAO-A and MAO-B, kynuramine, benzylamine, toloxatone, lazabemide, AChE (Type VI-S from Electrophorus electricus), BChE (equine serum), acetylthiocholine iodide (ATCI), butyrylthiocholine iodide (BTCI), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), tacrine, donepezil, and the BACE-1 activity detection kit were purchased from Sigma-Aldrich (St. Louis, MO, USA). Clorgyline and pargyline were obtained from BioAssay Systems (Hayward, CA, USA).
Roswell Park Memorial Institute-1640 medium (RPMI-1640), Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin solution were purchased from Hyclone Laboratories (San Ramon, CA, USA). The cell counting kit-8 (CCK-8) and dimethyl sulfoxide (DMSO) were obtained from Dojindo Laboratories (Kumamoto, Japan) and Sigma-Aldrich, respectively.

NMR experiments were conducted on a Unity INOVA 400 spectrometer (Varian, IL, USA). ¹H- and ¹³C-NMR spectra were recorded at 400 and 100 MHz, respectively, and tetramethylsilane was used as the internal standard. ESI MS analyses were performed on a Micromass QTQF2 mass spectrometer (Water, Milford, MA, USA). The untraviloet (UV) was measured with a Shimadzu UV-1800 UV-Vis spectrophotometer (Shimadzu, Japan). IR spectrum was measured with a Shimadzu IR-408 spectrophotometer in CHCl₃ solution (Shimadzu, Japan). TLC was carried out on silica gel F₂₅₄-precoated glass plates and RP-18 F_254S plates (Merck, Germany). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), trypsin-EDTA 0.25%, streptomycin and penicillin were obtained from Hyclone (Logan, UT, USA). Dimethyl sulfoxide (DMSO), and a Dojindo Kit was purchased from Dojindo Molecular Technology INC (Maryland, USA). Annexin V-FITC/PI double staining detection kit, mitochondrial membrane potential assay kit with caspase-3 activity assay kit, propidium iodide (PI) were purchased from Beyotime (Beyotime Institute of Biotechnology, Shanghai, China). All used chemicals and reagents were of analytical grade.