Renal tissue RNA was isolated from a small piece of flash-frozen tissue using the RNeasy Plus Mini Kit (Qiagen, Germantown, MD) and cDNA was synthesized using the qScript XLT cDNA SuperMix (Quantabio, Beverly, MA). For human cell RNA, RCTE, 9–12, and HCC827 cells were treated with IFN-γ (100 ng/ml) or phosphate-buffered saline solution (control) for 24 hours prior to RNA isolation. Quantitative polymerase chain reaction was performed using the PowerSYBER PCR Master Mix (Applied Biosystems) on a CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA). Primer details can be found in the Supplementary Methods .
Powersyber pcr master mix
Manufactured by Thermo Fisher Scientific
PowerSYBER PCR Master Mix is a ready-to-use solution for performing polymerase chain reaction (PCR) experiments. It contains all the necessary components, including a DNA polymerase, buffer, and fluorescent dye, to amplify target DNA sequences.
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Lab products found in correlation
2 protocols using powersyber pcr master mix
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Quantification of RNA Expression
2
Quantification of RNA Expression
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Renal tissue RNA was isolated from a small piece of flash-frozen tissue using the RNeasy Plus Mini Kit (Qiagen, Germantown, MD) and cDNA was synthesized using the qScript XLT cDNA SuperMix (Quantabio, Beverly, MA). For human cell RNA, RCTE, 9–12, and HCC827 cells were treated with IFN-γ (100 ng/ml) or phosphate-buffered saline solution (control) for 24 hours prior to RNA isolation. Quantitative polymerase chain reaction was performed using the PowerSYBER PCR Master Mix (Applied Biosystems) on a CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA). Primer details can be found in the Supplementary Methods .
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