Anti becn1 antibody

Manufactured by Santa Cruz Biotechnology

The Anti-BECN1 antibody is a laboratory reagent used to detect and study the BECN1 (Beclin 1) protein. BECN1 is a key regulator of autophagy, a cellular process involved in the degradation and recycling of damaged or unwanted cellular components. The antibody can be used in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to investigate the expression and localization of BECN1 in biological samples.

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Lab products found in correlation

Halt phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Halt proteinase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) One hour western detection kit, by GenScript (1 mentions) Anti gapdh antibody, by Merck Group (1 mentions) Anti flag antibody, by Merck Group (1 mentions) Anti mtor antibody, by Cell Signaling Technology (1 mentions) Anti gfp antibody, by Santa Cruz Biotechnology (1 mentions) Anti lc3b antibody, by Abcam (1 mentions) Anti gapdh antibody, by Proteintech (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Hoechst, by Merck Group (1 mentions) Horseradish peroxidase conjugated sheep anti mouse and anti rabbit antibodies, by Jackson ImmunoResearch (1 mentions) Anti p70s6k antibody, by Abcam (1 mentions) Anti lc3b antibody, by Novus Biologicals (1 mentions) Anti lamp2 antibody, by Santa Cruz Biotechnology (1 mentions) Anti tubulin antibody, by Proteintech (1 mentions) Alexa fluor 594 conjugated affinipure donkey anti mouse igg antibody, by Proteintech (1 mentions)

Spelling variants of this product

BECN1 (19 citations) Anti-BECN1 (8 citations)

2 protocols using anti becn1 antibody

BCL2 Immunoprecipitation from Mouse Tissues

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Mouse tissues (muscle and brain) were homogenized in lysis buffer containing 50 mM Tris (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, halt proteinase inhibitor cocktail (ThermoFisher Scientific), and halt phosphatase inhibitor cocktail (ThermoFisher Scientific), and subjected to immunoprecipitation with anti-BCL2 monoclonal antibody conjugated agarose beads (Santa Cruz Biotechnology 7382 AC). Eluates were separated by SDS-PAGE and detected by anti-BCL2-HRP antibody (C2 Santa Cruz Biotechnology, 1:500) and anti-BECN1 antibody (Santa Cruz Biotechnology, 1:200) using the ONE-HOUR Western Detection Kit (GenScript Corporation) following the manufacturer’s instruction.

Rocchi A., Yamamoto S., Ting T., Fan Y., Sadleir K., Wang Y., Zhang W., Huang S., Levine B., Vassar R, & He C. (2017). A Becn1 mutation mediates hyperactive autophagic sequestration of amyloid oligomers and improved cognition in Alzheimer's disease. PLoS Genetics, 13(8), e1006962.

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Antibody Panel for Protein Analysis

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The following primary antibodies were used: anti-FLAG antibody (Sigma), anti-GFP antibody (Santa Cruz), anti-GAPDH antibody (Millipore), anti-phospho-p70S6K (T389) antibody (Cell Signaling Technology), anti-p70S6K antibody (Epitomics), anti-mTOR antibody (Cell Signaling Technology), anti-LC3B antibody (Novus Biologicals), anti-LC3B antibody (Abcam), anti-GAPDH antibody (Proteintech), anti-Tubulin antibody (Proteintech), anti-MAP2 antibody (Proteintech), anti-LAMP2 antibody (Santa Cruz), anti-Bcl2 (total, pS70 or pS87) antibody (Santa Cruz) and anti-BECN1 antibody (Santa Cruz). The following secondary antibodies were used: horseradish peroxidase-conjugated sheep anti-mouse and anti-rabbit antibodies (Jackson ImmunoResearch Laboratories) for immunoblotting and Alexa Fluor 594-conjugated AffiniPure Donkey Anti-Mouse IgG antibody (Proteintech), Alexa Fluor 594-conjugated AffiniPure Donkey Anti-Rabbit IgG antibody (Proteintech) or Alexa Fluor 647-conjugated AffiniPure Donkey Anti-Rabbit IgG antibody (Yasen Biotechnology) for immunocytochemistry and immunohistochemistry. DAPI (Sigma) or Hoechst (Sigma) were used for nuclei staining.

Xu S., Ma Q., Shen J., Li N., Sun S., Wang N., Chen Y., Dong C., Tam K.Y., Prehn J.H., Wang H, & Ying Z. (2024). ALS-linked C9orf72 dipeptide repeats inhibit starvation-induced autophagy through modulating BCL2–BECN1 interaction. Acta Pharmaceutica Sinica. B, 14(5), 2026-2038.

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