Phospho-Histone H2AX (Ser139) (cat #9718S), phospho-p53 (Ser15) (cat #9284S), and GAPDH (D16H11) (cat #5174S) rabbit monoclonal primary antibodies (1:1000 dilution); and horseradish peroxidase conjugated anti-rabbit secondary antibody (7074S) (1:2000 dilution) were purchased from Cell Signaling Technology, Danvers, MA. Primary antibody incubations were carried out overnight at 4°C Secondary antibody incubations were carried out at room temperature for 1 hour. The Western blot signal was detected by Pierce ECL Western Blotting Substrate (Thermo Scientific, Rockford, IL) and developed onto Hyperfilm (GE Healthcare Life Sciences, Pittsburgh, PA) or imaged using Bio-Rad ChemiDoc Imager.
Horseradish peroxidase conjugated anti rabbit secondary antibody 7074s
Manufactured by Cell Signaling Technology
Horseradish peroxidase conjugated anti-rabbit secondary antibody (7074S) is a reagent used in western blotting and other immunoassays. It is a secondary antibody that binds to rabbit primary antibodies and is conjugated to the enzyme horseradish peroxidase, which can be used to detect and visualize the target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Phospho p53 ser15, by Cell Signaling Technology (1 mentions)
Phospho histone h2a x ser139, by Cell Signaling Technology (1 mentions)
Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)
Hyperfilm, by GE Healthcare (1 mentions)
Chemidoc imager, by Bio-Rad (1 mentions)
Gapdh d16h11, by Cell Signaling Technology (1 mentions)
Click it rna alexa fluor 594 imaging kit, by Thermo Fisher Scientific (1 mentions)
1 step human coupled ivt kit, by Thermo Fisher Scientific (1 mentions)
Syto rnaselect reagent, by Thermo Fisher Scientific (1 mentions)
1 6 hexanediol, by Tokyo Chemical Industry (1 mentions)
2 protocols using horseradish peroxidase conjugated anti rabbit secondary antibody 7074s
1
Western Blot Analysis of DNA Damage Markers
2
RNA Labeling and Imaging Workflow
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1-Step Human Coupled IVT kit, poly-rA RNA, Click-iT RNA Alexa Fluor 594 imaging kit, and SYTO-RNASelect reagent were purchased from Thermo Fisher Scientific. Recombinant human IPO7 (Ag27257), recombinant human GIT1 (Ag10658), rabbit polyclonal anti-NAP1L4 antibody (16018-1-AP), rabbit polyclonal anti-PAF1 antibody (15441-1-AP), rabbit polyclonal anti-IPO7 antibody (28289-1-AP), rabbit polyclonal anti-RPL5 antibody (15430-1-AP), and rabbit polyclonal anti-EIF3A antibody (11423-1-AP) were purchased from Proteintech. Horseradish peroxidase–conjugated anti-rabbit secondary antibody (#7074S) was purchased from Cell Signaling. Chambered coverglasses (uncoated or hydrophilic polymer MAS-coated) were obtained from Matsunami Glass. 1,6-Hexanediol was purchased from Tokyo Chemical Industry.
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