Genomic SARS-CoV-2 RNA (2019-nCoV/USA-WA1/2020, ATCC VR-1986D), HCoV-OC43 (ATCC VR-1558D) and H1N1 (ATCC VR-1736D) RNAs were purchased from LGC standards, UK. SARS-CoV-2 verification panel (AccuPlex SARS-CoV-2, 0505-0168) was supplied by Technopath, UK. LAMP primers and lateral flow reporter were purchased from Integrated DNA Technologies (IDT). SYBR Safe, nuclease-free water, RNase decontamination solution and PCR adhesive film were supplied by Thermofisher Scientific, UK. Guanidine hydrochloride (GuHCl) and MagneSil paramagnetic beads were purchased from Promega. Lateral flow strips (Milenia HybriDetect 1) were purchased from TwistDx, UK.
Magnesil paramagnetic beads
Manufactured by Promega
MagneSil paramagnetic beads are a type of magnetic bead used for various applications in molecular biology and biochemistry. These beads are made of a magnetic core coated with a silica-based surface, enabling their use in a wide range of procedures that involve the separation, purification, or manipulation of biomolecules, such as DNA, RNA, and proteins. The magnetic properties of these beads allow for efficient separation and washing steps, making them a versatile tool for researchers and scientists.
Automatically generated - may contain errors
Lab products found in correlation
Sybr safe, by Thermo Fisher Scientific (1 mentions)
Adhesive pcr film, by Thermo Fisher Scientific (1 mentions)
Nuclease free water, by Thermo Fisher Scientific (1 mentions)
Hcov oc43, by LGC (1 mentions)
Rnase decontamination solution, by Thermo Fisher Scientific (1 mentions)
Lamp primers, by Integrated DNA Technologies (1 mentions)
Lateral flow strips milenia hybridetect 1, by Twist Bioscience (1 mentions)
2 protocols using magnesil paramagnetic beads
1
SARS-CoV-2 Detection Using LAMP and Lateral Flow
2
Magnetic Bead-based RNA Extraction
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A magnetic bead-based extraction method was used for all contrived samples13 (link). Extraction was performed by spiking 15 μL of MagneSil paramagnetic beads (Promega) into the mixture and incubating at room temperature for 5 minutes with intermittent tube inversion to maintain suspension. Magnetic beads were collected on a magnetic stand rack and supernatant was removed from the beads. The beads were washed twice with 600 μL 70% ethanol and air-dried for 10 minutes at room temperature. RNA was eluted in 25 μL nuclease-free water. After a 2-minute incubation, beads were separated on a magnetic rack and the supernatant was transferred to a clean 0.6 mL tube (Genesee Scientific) and stored at -80 °C until use.
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