Cytokine arrays

Manufactured by R&D Systems

Sourced in United States

Cytokine arrays are a type of lab equipment used to detect and quantify multiple cytokines or chemokines in a single biological sample. They typically consist of a solid-state substrate, such as a membrane or microplate, on which capture antibodies specific to different cytokines are immobilized. The arrays allow for the simultaneous measurement of a panel of cytokines, providing a comprehensive profile of the cytokine environment within the sample.

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Mouse XL Cytokines Array Kit Rat cytokines array panel A Human cytokine protein arrays Mouse cytokine arrays Cytokine antibody arrays Human cytokine arrays Proteome Profiler Human Cytokine Array (Panel A Kit, Human cytokine array panel A membranes Proteome Profiler Human Cytokine Array Rat Cytokine Array Panel A

3 protocols using cytokine arrays

Multiplex Cytokine Profiling in Plasma

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Cytokine Arrays (kit ARY005, R&D systems, Minneapolis, USA) were performed using human plasma samples collected from peripheral blood after centrifugation at 1,000 × g for 30 min in an EDTA tube. The plasma sample was pre-mixed with a detection antibody cocktail and applied to the blocked array membrane. Signals from different plasma samples reacting with membranes within the same time were detected simultaneously. Arrays were conducted according to the manufacturer's instructions, and allowed the parallel determination of 36 cytokines. In this study, D5-D10 immunoblots were adapted from original membrane spots.

Wang H.M., Lin T.L., Kuo Y.C., Li H.P., Chang K.P., Lin C.Y., Fan H.C., Lin A.C., Hsieh C.H., Tsang N.M., Lee L.Y., Chan S.C., Lui K.W., Chang Y.S, & Hsu C.L. (2016). Correlation between overall survival and differential plasma and tissue tumor marker expression in nasopharyngeal carcinoma patients with different sites of organ metastasis. Oncotarget, 7(33), 53217-53229.

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Cytokine Profiling of Murine Aortic Tissue

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Using a cytokine array, 40 cytokines were screened in the sample aortic tissue. Using isolated protein from murine abdominal aortas, cytokine arrays (R&D Systems, Minneapolis, MN) were completed according to manufacturer instructions. Protein samples from each group were pooled for analysis, all samples were run in duplicate, and the mean value was used as described previously.^{18 (link)}

Montgomery W.G., Spinosa M.D., Cullen J.M., Salmon M.D., Su G., Hassinger T., Sharma A.K., Lu G., Fashandi A., Ailawadi G, & Upchurch GR J.r. (2018). Tamsulosin Attenuates Abdominal Aortic Aneurysm Growth. Surgery, 164(5), 1087-1092.

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Cytokine Profiling in Murine Aortas

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Using isolated protein from murine abdominal aortas, cytokine arrays (R&D Systems, 7 Minneapolis, MN) were completed according to manufacturer instructions, on mice harvested on 8 days 3 and 28 (n=4–6/group). Protein samples from each group were pooled for analysis, and all 9 samples were run in duplicate, and the mean value was used.^{21 (link)}

Spinosa M., Su G., Salmon M., Lu G., Cullen J.M., Fashandi A.Z., Hawkins R.B., Montgomery W., Meher A.K., Conte M.S., Sharma A.K., Ailawadi G, & Upchurch GR J.r. (2018). Resolvin D1 Decreases Abdominal Aortic Aneurysm Formation by Inhibiting NETosis in a Mouse Model. Journal of vascular surgery, 68(6 Suppl), 93S-103S.

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