Rat anti cd8

Manufactured by BioLegend

Rat anti-CD8 is a monoclonal antibody that recognizes the CD8 cell surface antigen, which is expressed on a subset of T cells. It can be used for the identification and enumeration of CD8+ T cells in flow cytometric analysis.

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Lab products found in correlation

Rabbit anti cd3, by Abcam (2 mentions) Hrp conjugated secondary antibody, by Vector Laboratories (2 mentions) 3 3 diaminobenzidine (dab), by Vector Laboratories (2 mentions) Hamsteranti cd11c, by BioLegend (2 mentions) Goat anti rat igg, by Vector Laboratories (2 mentions) Hematoxylin, by StatLab (2 mentions) Horse anti rabbit igg, by Vector Laboratories (2 mentions) Goatanti hamster igg, by Vector Laboratories (2 mentions) Lsm 700, by Zeiss (1 mentions) Streptavidin biotin blocking kit, by Vector Laboratories (1 mentions) Alexa fluor 594 labeled secondary antibody, by Thermo Fisher Scientific (1 mentions) Rat anti 1 a 1 e, by BioLegend (1 mentions) Antifade mountant with dapi, by Vector Laboratories (1 mentions) Guinea pig anti insulin, by Agilent Technologies (1 mentions) Anti rat cd3, by BioLegend (1 mentions) Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Zen software, by Zeiss (1 mentions) Oct mounting medium, by Avantor (1 mentions) Lsm 700 confocal microscope, by Zeiss (1 mentions)

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Anti-CD8 (141 citations)

5 protocols using rat anti cd8

Immunohistochemical Analysis of Vaginal Immune Cells

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The vagina was embedded in OCT and snap frozen in liquid nitrogen
pre-cooled isopentane. Frozen vaginal tissue blocks were cut into sections with
the thickness of 5 μm, stored in −70°C until use.
Cryostat sections were fixed in acetone for 5 min, rehydrated in PBS, and
incubated overnight with primary antibodies including rabbit anti-CD3 (1:100
dilution, AbCam), rat anti-CD8 (1:200 dilution, Biolegend) and hamster
anti-CD11c (1:150 dilution, Biolegend). The primary antibodies were washed out 3
times by PBS followed by the addition of HRP conjugated secondary antibodies
(Vector Lab) including horse anti-rabbit IgG, goat anti-rat IgG and goat
anti-hamster IgG. The secondary antibodies were incubated in room temperature
for 30 minutes followed by DAB (kit from Vector Lab) development and hematoxylin
(BBC Biochemical) counter staining.

Wang Y., Sui Y., Kato S., Hogg A.E., Steel J.C., Morris J.C, & Berzofsky J.A. (2015). Vaginal type-II mucosa is an inductive site for primary CD8+ T-cell mucosal immunity. Nature communications, 6, 6100.

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Immunohistochemical Analysis of Vaginal Immune Cells

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Immunofluorescence Analysis of Skin Infiltrates

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Inflammatory skin infiltrates and markers were evaluated on frozen skin sections as previously described (4 (link)). Acetone-fixed frozen skin sections were blocked in Dulbecco’s PBS + 5% goat serum (catalog S-1000-20, Vector Laboratories) for 1 hour at room temperature. The fixed skin sections were incubated with rat anti-CD8 (catalog 100702, 1:100, BioLegend) and rat anti–I-A/I-E (catalog 107602, 1:100, BioLegend) overnight at 4°C, followed by incubation with Alexa Fluor 594–labeled secondary antibody (catalog A-11007, 1:500, Thermo Fisher Scientific). The endogenous biotin was blocked using a streptavidin/biotin blocking kit (catalog SP-2002, Vector Laboratories). Antifade Mountant with DAPI (catalog H-1200-10, Vector Laboratories) was used as the mounting medium. Immunofluorescence images were captured on a Zeiss LSM 700 laser scanning confocal microscope.

Dai Z., Chen J., Chang Y, & Christiano A.M. (2021). Selective inhibition of JAK3 signaling is sufficient to reverse alopecia areata. JCI Insight, 6(7), e142205.

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Immunohistochemical analysis of mouse pancreas

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Livers and pancreata removed from sacrificed mice were fixed in 4% PFA for 5 h at 4 °C, dehydrated in 30% sucrose overnight at 4 °C, and embedded in O.C.T mounting medium (VWR Chemicals) before freezing at −70 °C. Five micrometre cryostat (Leica) sections were air-dried, fixed in acetone for 6 min, air-dried, and stored at −20 °C. Immunolabelling was performed on sections pre-blocked with 1% BSA by incubating with rabbit anti glucagon (Millipore, cat no. AB932, 1:20) guinea pig anti-insulin (DAKO, cat no. A0564, 1:150), rabbit anti C-peptide (Cell Signalling, cat no. 4593S, 1:50), Rat anti-CD3 (Biolegend, cat no. 100202, 1:100), Rat anti-CD8 (Biolegend, cat no. 100702, 1:50) for 1 h at 4 °C. Incubation with relevant Alexa Fluor® secondary antibodies (Invitrogen, 1:500) and DAPI (4′,6-diamidino-2-phenylindole) (Invitrogen) followed. Sections were then mounted in Prolong Diamond Antifade solution (Thermo Fisher Scientific) and visualised using a Zeiss LSM 700 confocal microscope (Zeiss, Germany). Images were captured and processed using Zen software (Zeiss).

Recino A., Gan S.U., Sia K.C., Sawyer Y., Trendell J., Kay R., Gribble F.M., Reimann F., Foale R., Notaridou M., Holmes N., Lever A., Lee K.O., Nathwani A., Cooke A., Calne R, & Wallberg M. (2018). Immunosuppression overcomes insulin- and vector-specific immune responses that limit efficacy of AAV2/8-mediated insulin gene therapy in NOD mice. Gene Therapy, 26(1), 40-56.

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Immunohistochemistry Protocol for Tissue Staining

Cited 1 time

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As previously described(Yilmaz et al., 2012 (link)), tissues were fixed in 10% formalin, paraffin embedded and sectioned. Antigen retrieval was performed with Borg Decloaker RTU solution (Biocare Medical) in a pressurized Decloaking Chamber (Biocare Medical) for 3 minutes. Antibodies used: rat anti-BrdU (1:2000, Abcam 6326), mouse monoclonal β-catenin (1:100, BD Biosciences 610154), rabbit monoclonal OLFM4 (1:10,000, gift from CST, clone PP7), rat anti-CD3 (1:200, eBioscience, clone 145–2C11), rat anti-CD4, (1:200, Biolegend, clone H129.19), rat anti-CD8 (1:200, Biolegend, clone 53–6.7), anti-Ki67 (1:200, Thermo Fisher, MA5–14520). Biotin-conjugated secondary donkey anti-rabbit or anti-rat antibodies were used from Jackson ImmunoResearch. The Vectastain Elite ABC immunoperoxidase detection kit (Vector Labs) followed by Dako Liquid DAB+ Substrate (Dako) was used for visualization. All antibody incubations involving tissue or sorted cells were performed with Common Antibody Diluent (Cell Signaling).

Beyaz S., Chung C., Mou H., Bauer-Rowe K.E., Xifaras M.E., Ergin I., Dohnalova L., Biton M., Shekhar K., Eskiocak O., Papciak K., Ozler K., Almeqdadi M., Yueh B., Fein M., Annamalai D., Valle-Encinas E., Erdemir A., Dogum K., Shah V., Alici-Garipcan A., Meyer H.V., Özata D.M., Elinav E., Kucukural A., Kumar P., McAleer J.P., Fox J.G., Thaiss C.A., Regev A., Roper J., Orkin S.H, & Yilmaz Ö.H. (2021). Dietary suppression of MHC-II expression in intestinal epithelial cells enhances intestinal tumorigenesis. Cell stem cell, 28(11), 1922-1935.e5.

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