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Ck40 32ph

Manufactured by Olympus
Sourced in Japan

The CK40-32PH is a compact and durable inverted microscope designed for routine laboratory applications. It features phase contrast optics, providing high-contrast images for examining transparent specimens. The microscope is equipped with a built-in Koehler illumination system and a reliable mechanical stage for precise specimen positioning.

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3 protocols using ck40 32ph

1

Erythrocyte Phagocytosis by Microglia

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Fresh blood was harvested from C57BL/6 mice by cardiac puncture as previously described [37 ]. After separation and purification of erythrocytes, they were counted and co-cultured with microglia at a ratio of 1:40 (microglia: erythrocytes) for 1 h. The ratio of microglia to erythrocytes refers to the fluorescent bioparticles phagocytosis assay. Microglia swallowing erythrocytes were observed using a microscope (Olympus CK40–32 PH, Japan). Three visual fields per sample were randomly selected and observed. The phagocytic index was measured as described previously.
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2

Histological Analysis of Oxidative Stress

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Portions of dorsal skin samples were fixed in 4% paraformaldehyde and embedded in paraffin. Sections (5 µm) were then cut and stained with hematoxylin and eosin (H&E). For immunohistochemical staining, sections were incubated overnight at 4°C with primary antibodies (anti-8-OHdG, anti-Bax, and antioccludin), washed, and incubated with HRP-conjugated antibodies for 1 h at room temperature. Peroxidase activities were determined using an AEC chromogen kit (AEC101, Sigma-Aldrich, St. Louis, MO, USA), and a digital camera (Olympus UC30, Japan) mounted on a phase-contrast microscope (Olympus CK40-32PH, Japan) using DIXI image solution 2.89 software (DIXI Optics, Daejeon, South Korea).
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3

Histological Analysis of Skin Samples

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For histopathological observation, a portion of the skin samples from the back in each group was fixed in 10% formalin for 24 h at 4°C. After paraffin embedding, sections were cut and stained with hematoxylin and eosin (H/E) or toluidine blue (TB) for detection of infiltrated inflammatory cells or mast cells, respectively. For immunohistochemical staining, sections were incubated overnight at 4°C with the indicated primary antibodies. After sections were washed, they were incubated with horseradish peroxidase- (HRP-) conjugated anti-rabbit antibodies or HRP-conjugated anti-mouse antibodies for 1 h at room temperature. Peroxidase activity was visualized with an AEC chromogen kit (Sigma-Aldrich, St. Louis, MO, USA) and examined using a digital camera (Olympus UC30, Japan) mounted on a phase contrast microscope (Olympus CK40-32PH, Japan) using DIXI image solution 2.89 software (DIXI Optics, Daejeon, South Korea).
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