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Dm lfs microscope

Manufactured by Leica
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The Leica DM-LFS is a high-performance microscope designed for laboratory settings. It is equipped with advanced optics and features that enable accurate observation and analysis of samples. The core function of the Leica DM-LFS is to provide a versatile platform for microscopic examination and investigation.

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Lab products found in correlation

Vts 1000 vibrating blade microtome, by Leica (2 mentions) Pclamp 10, by Molecular Devices (1 mentions) Cooled ccd camera, by Hamamatsu Photonics (1 mentions) Axopatch 200b amplifier, by Molecular Devices (1 mentions) Hcimage acquisition software, by Hamamatsu Photonics (1 mentions) Vt1000, by Leica (1 mentions)

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DM-LFS (21 citations)

7 protocols using dm lfs microscope

1

Visualizing ChR2 Expression in mPFC and NAcc

Cited 1 time
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Fifteen to 43 days following ChR2 virus injection, brain slices containing mPFC and/or NAcc were prepared as previously described36 (link). Briefly, animals were decapitated under isoflurane anesthesia and brains rapidly removed and immersed in ice cold cutting solution perfused with 95% oxygen-5% carbon dioxide. Two-hundred μm-thick coronal sections containing mPFC and/or NAcc were then cut using a VTS-1000 vibrating blade microtome (Leica Microsystems). Slices were kept in oxygenated cutting solution at 32° C for 1 hour before being transferred to a recording chamber with regular artificial cerebrospinal fluid (aCSF). Slices were imaged using a Leica DM-LFS microscope (Leica Microsystems) captured with SimplePCI software (Hamamatsu Corp.) for areas of strong fluorescence within the target region of interest (mPFC or NAcc), and recordings performed as follows:
Amadei E.A., Johnson Z.V., Kwon Y.J., Shpiner A.C., Saravanan V., Mays W.D., Ryan S.J., Walum H., Rainnie D.G., Young L.J, & Liu R.C. (2017). Dynamic corticostriatal activity biases social bonding in monogamous female prairie voles. Nature, 546(7657), 297-301.
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2

Acute OHC Preparation and Imaging

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To study OHCs in acutely dissected organs of Corti, mice were killed by cervical dislocation, the cochlea removed and the organ of Corti dissected in extracellular solution composed of (in mmol/L): 135 NaCl, 5.8 KCl, 1.3 CaCl2, 0.9 MgCl2, 0.7 NaH2PO4, 5.6 d‐glucose, 10 HEPES‐NaOH, 2 Na‐pyruvate. Amino acids and vitamins (Eagle's MEM) were added from concentrates (pH 7.5, 308 mOsm/kg). Once dissected, the apical and basal coils of the organ of Corti were transferred to a microscope chamber containing extracellular solution and viewed on a Leica DMLFS microscope (Leica Micro Systems, Wetzlar, Germany) through a long working‐distance 63× water‐immersion objective.
Corns L.F, & Marcotti W. (2016). Piezo1 haploinsufficiency does not alter mechanotransduction in mouse cochlear outer hair cells. Physiological Reports, 4(3), e12701.
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3

Visualizing ChR2 Expression in mPFC and NAcc

Cited 1 time
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Fifteen to 43 days following ChR2 virus injection, brain slices containing mPFC and/or NAcc were prepared as previously described36 (link). Briefly, animals were decapitated under isoflurane anesthesia and brains rapidly removed and immersed in ice cold cutting solution perfused with 95% oxygen-5% carbon dioxide. Two-hundred μm-thick coronal sections containing mPFC and/or NAcc were then cut using a VTS-1000 vibrating blade microtome (Leica Microsystems). Slices were kept in oxygenated cutting solution at 32° C for 1 hour before being transferred to a recording chamber with regular artificial cerebrospinal fluid (aCSF). Slices were imaged using a Leica DM-LFS microscope (Leica Microsystems) captured with SimplePCI software (Hamamatsu Corp.) for areas of strong fluorescence within the target region of interest (mPFC or NAcc), and recordings performed as follows:
Amadei E.A., Johnson Z.V., Kwon Y.J., Shpiner A.C., Saravanan V., Mays W.D., Ryan S.J., Walum H., Rainnie D.G., Young L.J, & Liu R.C. (2017). Dynamic corticostriatal activity biases social bonding in monogamous female prairie voles. Nature, 546(7657), 297-301.
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4

Visualizing SCN Neuron Electrophysiology

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SCN neurons were visualized using a Leica DMLFS microscope (Leica Microsystems, Buffalo Grove, IL, USA) equipped with near-infrared (IR)-differential interference contrast and fluorescence optics. For loose-patch recordings, patch electrodes (4–6 MΩ) pulled from glass capillaries (WPI) on a multistage puller (DMZ; Zeitz, Martinsried, Germany) were filled with extracellular solution. Spontaneous action potential recordings (∼5 min in duration) from neurons sampled throughout the SCN were obtained with an Axopatch 200 B amplifier (Molecular Devices, Sunnyvale, CA, USA) and monitored online with pClamp 10.0 software (Molecular Devices). Recordings obtained in gap-free mode throughout the day were sampled at 10 kHz, and were filtered online at 1 kHz. Loose-patch seal resistances ranged from 10–30 MΩ. Slices were used for no more than 6 h after dissection. Immediately after cessation of electrophysiological recording, an image of the recorded neuron was captured with an exposure time of one second using HCImage acquisition software (Hamamatsu Photonics, Bridgewater, NJ, USA) with a cooled CCD camera (Hamamatsu) and an EGFP filter set. All recordings were confirmed to be from GFP+ neurons by aligning digital images of the same neuron taken under near-IR and GFP fluorescence illumination.
Jones J.R, & McMahon D.G. (2016). The core clock gene Per1 phases molecular and electrical circadian rhythms in SCN neurons. PeerJ, 4, e2297.
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5

Callose Staining and Quantification

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Callose staining was carried out as previously described44 . Seedlings were destained overnight before aniline blue stain was applied for 3 h in the dark. Callose images were obtained from cotyledons 10 days post-germination treated for 12 h with 1 µm flg22 or water. Cotyledons were mounted in 50% glycerol and imaged using a LEICA DMLFS microscope with a 5x objective lens and UV D filter. Callose was quantified using an image processing macro in FIJI45 (link) adapted from46 (link). Colour images were converted to 8-bit greyscale before analysis.
Hurst C.H., Wright K.M., Turnbull D., Leslie K., Jones S, & Hemsley P.A. (2019). Juxta-membrane S-acylation of plant receptor-like kinases is likely fortuitous and does not necessarily impact upon function. Scientific Reports, 9, 12818.
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6

Hypothalamic Slice Preparation and Recording

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Sprague Dawley rats (20–24 days old) were deeply anesthetized with halothane, their brains removed and submerged in ice-cold artificial cerebrospinal fluid (ASCF) containing (in mM): NaCl 87, KCl 2.5, NaH2PO4 1.25, NaHCO3 25, MgCl2 7, CaCl2 0.5, glucose 25, and sucrose 75 saturated with 95% O2 / 5% CO2. Coronal (250 μm) slices containing the hypothalamus were prepared on a vibratome (Leica VT 1000S). Slices were transferred first to a holding chamber in which the temperature was gradually raised to 35°C over 30 min. Then, individual slices were transferred to the recording chamber in which they were completely submerged in 32° C ACSF (same as above, except MgCl2 1.2, CaCl2 2.4 mM). Experiments were performed with cells visualized using infrared differential interference contrast microscopy (IR-DIC, Leica DMLFS microscope).
Matus-Ortega M.E., Gelman P.L., Calva-Nieves J.C., Flores-Zamora A., Salazar-Juárez A., Torner-Aguilar C.A., Gamba G., De Los Heros P., Peng B., Pintar J.E., Gompf H.S., Allen C.N, & Antón-Palma B. (2017). Mexneurin is a novel precursor of peptides in the central nervous system of rodents. FEBS letters, 591(12), 1627-1636.
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7

Cortical Neuron Electrophysiology Protocol

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Slices were transferred to a recording chamber, maintained at 30-32 °C, and perfused with oxygenated regular artificial cerebrospinal fluid (ACSF) at 1 ml/min. Neurons in the mPFC were visualized using two water immersion objectives (HCX/APO L 10X/0.30 and 40X/0.80) with infrared differential interference contrast (DMLFS microscope, Leica, Wetzlar, Germany) connected with an infraredsensitive camera. For details, see the Supplementary Information.
Leggio G.M., Torrisi S.A., Mastrogiacomo R., Mauro D., Chisari M., Devroye C., Scheggia D., Nigro M., Geraci F., Pintori N., Giurdanella G., Costa L., Bucolo C., Ferretti V., Sortino M.A., Ciranna L., De Luca M.A., Mereu M., Managò F., Salomone S., Drago F, & Papaleo F. (2021). The epistatic interaction between the dopamine D3 receptor and dysbindin-1 modulates higher-order cognitive functions in mice and humans. Molecular psychiatry, 26(4).
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