Polyclonal rabbit anti human β actin

Total RNA was isolated from cells using TRIzol Reagent (Invitrogen), and then complementary DNA (cDNA) was synthesized using AMV reverse transcriptase (Promega, Madison, WI, USA) according to the manufacturer's instructions. The cDNA was used as a template for quantitative real-time PCR using an ABI Prism 7500 real-time PCR instrument (Applied Biosystems, Carlsbad, CA, USA). The primers used for real-time quantitative PCR are listed in Supplementary Table 1.
For western blotting analysis, total protein was prepared from human liver cell lines and clinical hepatocellular carcinoma tissue samples. Immunoblotting was performed according to standard procedures with polyclonal rabbit anti-human LDHA, anti-human c-Myc, anti-α-Tubulin (Cell Signaling, Bedford, MA, USA), monoclonal mouse anti-human NDRG2 (Abnova, Taipei, Taiwan), and polyclonal rabbit anti-human β-actin (Biosynthesis Biotechnology, Beijing, China) antibodies.

Total RNA was isolated from cells using Trizol Reagent (Invitrogen), and then complementary DNA (cDNA) was synthesized using AMV reverse transcriptase (Promega, Madison, WI, USA) according to the manufacturer's instructions. The cDNA was used as a template for quantitative real-time PCR using the ABI Prism 7500 real-time PCR instrument (Applied Biosystems, Carlsbad, CA, USA). The primers used in real-time quantitative PCR are shown in Supplementary Table S3.
For Western blot analysis, total protein was prepared from cell lines and clinical colorectal carcinomas tissue samples. Immunoblotting were performed according to standard procedures with polyclonal rabbit anti-human HK2, PKM2, LDHA, ASCT2, c-Myc, β-catenin (Cell Signaling, Bedford, MA, USA), polyclonal rabbit anti-human GLS1 (Abcam, Cambridge, UK), monoclonal mouse anti-human GLUT1 (Abcam, Cambridge, UK), monoclonal mouse anti-human NDRG2 (Abnova, Taipei, Taiwan), and polyclonal rabbit anti-human β-actin (Biosynthesis Biotechnology, Beijing, China) antibody.